Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli and in tobacco BY-2 cells | Nicotiana tabacum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Nicotiana tabacum | 5829 | - |
nucleus | - |
Nicotiana tabacum | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | inhibits activity | Nicotiana tabacum | |
Li+ | inhibits activity | Nicotiana tabacum | |
Mg2+ | required | Nicotiana tabacum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
GST-NtGPPase fusion protein | Nicotiana tabacum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0035 | - |
substrate: myo-inositol 1-phosphate, pH 7.5, 30°C | Nicotiana tabacum |
0.0376 | - |
substrate: L-galactose 1-phosphate, pH 7.5, 30°C | Nicotiana tabacum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-galactose 1-phosphate + H2O | - |
Nicotiana tabacum | L-galactose + phosphate | - |
? | |
additional information | enzyme also dephosphorylates myo-inositol 1-phosphate | Nicotiana tabacum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
L-galactose-1-phosphate phosphatase | - |
Nicotiana tabacum |
NtGPPase | - |
Nicotiana tabacum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Nicotiana tabacum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Nicotiana tabacum |
General Information | Comment | Organism |
---|---|---|
malfunction | tobacco BY-2 cells over-expressing NtGPPase have a higher content of L-ascorbic acid 13 days after subculture compared to control cells | Nicotiana tabacum |