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Literature summary for 3.1.3.B9 extracted from
- Molecular cloning and characterization of L-galactose-1-phosphate phosphatase from tobacco (Nicotiana tabacum) (2012), Biosci. Biotechnol. Biochem., 76, 1155-1162.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli and in tobacco BY-2 cells | Nicotiana tabacum |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Nicotiana tabacum | 5829 | - |
| nucleus | - |
Nicotiana tabacum | 5634 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | inhibits activity | Nicotiana tabacum |  |
| Li+ | inhibits activity | Nicotiana tabacum | |
| Mg2+ | required | Nicotiana tabacum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | - |
GST-NtGPPase fusion protein | Nicotiana tabacum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Nicotiana tabacum | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0035 | - |
substrate: myo-inositol 1-phosphate, pH 7.5, 30°C | Nicotiana tabacum |
| 0.0376 | - |
substrate: L-galactose 1-phosphate, pH 7.5, 30°C | Nicotiana tabacum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-galactose 1-phosphate + H2O | - |
Nicotiana tabacum | L-galactose + phosphate | - |
? | |
| additional information | enzyme also dephosphorylates myo-inositol 1-phosphate | Nicotiana tabacum | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| L-galactose-1-phosphate phosphatase | - |
Nicotiana tabacum |
| NtGPPase | - |
Nicotiana tabacum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Nicotiana tabacum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Nicotiana tabacum |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | tobacco BY-2 cells over-expressing NtGPPase have a higher content of L-ascorbic acid 13 days after subculture compared to control cells | Nicotiana tabacum |
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