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Literature summary for 3.1.3.B9 extracted from

  • Laing, W.A.; Bulley, S.; Wright, M.; Cooney, J.; Jensen, D.; Barraclough, D.; MacRae, E.
    A highly specific L-galactose-1-phosphate phosphatase on the path to ascorbate biosynthesis (2004), Proc. Natl. Acad. Sci. USA, 101, 16976-16981.
    View publication on PubMed
Search for more literature for 3.1.3.B9

Cloned(Commentary)

Cloned (Comment) Organism
transformed into Escherichia coli DH5alpha cells Actinidia deliciosa

Inhibitors

Inhibitors Comment Organism Structure
LiCl
-
 Arabidopsis thaliana
Mg2+ above 0.2 mM Actinidia deliciosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.022
-
 L-galactose 1-phosphate pH 7.0, 30°C, 4.8 mM MgCl2 Actinidia deliciosa
0.041
-
 L-galactose 1-phosphate pH 7.0, 30°C, 1.8 mM MgCl2 Actinidia deliciosa
0.15
-
 L-galactose 1-phosphate pH 7.0, 30°C Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ the enzyme is completely dependent on Mg2+ for activity. Ka(Mg2+): 0.2 mM Actinidia deliciosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29121
-
 2 * 29121, calculated from sequence Arabidopsis thaliana
65000
-
-
 Actinidia deliciosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-galactose 1-phosphate + H2O Actinidia deliciosa
-
 L-galactose + phosphate
-
 ?
L-galactose 1-phosphate + H2O Arabidopsis thaliana the enzyme is involved in biosynthesis of asorbate L-galactose + phosphate
-
 ?
L-galactose 1-phosphate + H2O Actinidia deliciosa A. Chev.
-
 L-galactose + phosphate
-
 ?
L-galactose 1-phosphate + H2O Arabidopsis thaliana Columbia-1 the enzyme is involved in biosynthesis of asorbate L-galactose + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Actinidia deliciosa Q5U789
-
-
Actinidia deliciosa A. Chev. Q5U789
-
-
Arabidopsis thaliana Q9M8S8
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Actinidia deliciosa

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
 Actinidia deliciosa
-
fruit
-
 Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
16.4
-
 pH 7.0, 30°C Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-galactose 1-phosphate + H2O
-
 Actinidia deliciosa L-galactose + phosphate
-
 ?
L-galactose 1-phosphate + H2O the enzyme is involved in biosynthesis of asorbate Arabidopsis thaliana L-galactose + phosphate
-
 ?
L-galactose 1-phosphate + H2O the enzyme is very specific for L-galactose 1-phosphate Actinidia deliciosa L-galactose + phosphate
-
 ?
L-galactose 1-phosphate + H2O the enzyme is very specific for L-galactose 1-phosphate Arabidopsis thaliana L-galactose + phosphate
-
 ?
L-galactose 1-phosphate + H2O
-
 Actinidia deliciosa A. Chev. L-galactose + phosphate
-
 ?
L-galactose 1-phosphate + H2O the enzyme is very specific for L-galactose 1-phosphate Actinidia deliciosa A. Chev. L-galactose + phosphate
-
 ?
L-galactose 1-phosphate + H2O the enzyme is involved in biosynthesis of asorbate Arabidopsis thaliana Columbia-1 L-galactose + phosphate
-
 ?
L-galactose 1-phosphate + H2O the enzyme is very specific for L-galactose 1-phosphate Arabidopsis thaliana Columbia-1 L-galactose + phosphate
-
 ?
additional information the protein shows some myo-inositol-1-Pase activity Arabidopsis thaliana ?
-
 ?
additional information the protein shows some myo-inositol-1-Pase activity Arabidopsis thaliana Columbia-1 ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 29121, calculated from sequence Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
L-Gal-1-Pase
-
 Actinidia deliciosa
L-Gal-1-Pase
-
 Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Actinidia deliciosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Actinidia deliciosa
7
-
-
 Arabidopsis thaliana

pH Range

pH Minimum pH Maximum Comment Organism
6.2 7.5 pH 6.2: about 20% of maximal activity, pH 7.5: about 35% of maximal activity Actinidia deliciosa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.7
-
 LiCl pH 7.0, 30°C Arabidopsis thaliana

pI Value

Organism Comment pI Value Maximum pI Value
Arabidopsis thaliana calculated from sequence
-
 5.01