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Literature summary for 3.1.3.B9 extracted from

  • Laing, W.A.; Bulley, S.; Wright, M.; Cooney, J.; Jensen, D.; Barraclough, D.; MacRae, E.
    A highly specific L-galactose-1-phosphate phosphatase on the path to ascorbate biosynthesis (2004), Proc. Natl. Acad. Sci. USA, 101, 16976-16981.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.B9

Cloned(Commentary)

Cloned (Comment) Organism
transformed into Escherichia coli DH5alpha cells Actinidia deliciosa

Inhibitors

Inhibitors Comment Organism Structure
LiCl
-
 Arabidopsis thaliana
Mg2+ above 0.2 mM Actinidia deliciosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.022
-
 L-galactose 1-phosphate pH 7.0, 30°C, 4.8 mM MgCl2 Actinidia deliciosa
0.041
-
 L-galactose 1-phosphate pH 7.0, 30°C, 1.8 mM MgCl2 Actinidia deliciosa
0.15
-
 L-galactose 1-phosphate pH 7.0, 30°C Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ the enzyme is completely dependent on Mg2+ for activity. Ka(Mg2+): 0.2 mM Actinidia deliciosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29121
-
 2 * 29121, calculated from sequence Arabidopsis thaliana
65000
-
-
 Actinidia deliciosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-galactose 1-phosphate + H2O Actinidia deliciosa
-
 L-galactose + phosphate
-
 ?
L-galactose 1-phosphate + H2O Arabidopsis thaliana the enzyme is involved in biosynthesis of asorbate L-galactose + phosphate
-
 ?
L-galactose 1-phosphate + H2O Arabidopsis thaliana Columbia-1 the enzyme is involved in biosynthesis of asorbate L-galactose + phosphate
-
 ?
L-galactose 1-phosphate + H2O Actinidia deliciosa A. Chev.
-
 L-galactose + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Actinidia deliciosa Q5U789
-
-
Actinidia deliciosa A. Chev. Q5U789
-
-
Arabidopsis thaliana Q9M8S8
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Actinidia deliciosa

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
 Arabidopsis thaliana
-
fruit
-
 Actinidia deliciosa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
16.4
-
 pH 7.0, 30°C Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-galactose 1-phosphate + H2O
-
 Actinidia deliciosa L-galactose + phosphate
-
 ?
L-galactose 1-phosphate + H2O the enzyme is involved in biosynthesis of asorbate Arabidopsis thaliana L-galactose + phosphate
-
 ?
L-galactose 1-phosphate + H2O the enzyme is very specific for L-galactose 1-phosphate Arabidopsis thaliana L-galactose + phosphate
-
 ?
L-galactose 1-phosphate + H2O the enzyme is very specific for L-galactose 1-phosphate Actinidia deliciosa L-galactose + phosphate
-
 ?
L-galactose 1-phosphate + H2O the enzyme is involved in biosynthesis of asorbate Arabidopsis thaliana Columbia-1 L-galactose + phosphate
-
 ?
L-galactose 1-phosphate + H2O the enzyme is very specific for L-galactose 1-phosphate Arabidopsis thaliana Columbia-1 L-galactose + phosphate
-
 ?
L-galactose 1-phosphate + H2O
-
 Actinidia deliciosa A. Chev. L-galactose + phosphate
-
 ?
L-galactose 1-phosphate + H2O the enzyme is very specific for L-galactose 1-phosphate Actinidia deliciosa A. Chev. L-galactose + phosphate
-
 ?
additional information the protein shows some myo-inositol-1-Pase activity Arabidopsis thaliana ?
-
 ?
additional information the protein shows some myo-inositol-1-Pase activity Arabidopsis thaliana Columbia-1 ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 29121, calculated from sequence Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
L-Gal-1-Pase
-
 Arabidopsis thaliana
L-Gal-1-Pase
-
 Actinidia deliciosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Actinidia deliciosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Arabidopsis thaliana
7
-
-
 Actinidia deliciosa

pH Range

pH Minimum pH Maximum Comment Organism
6.2 7.5 pH 6.2: about 20% of maximal activity, pH 7.5: about 35% of maximal activity Actinidia deliciosa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.7
-
 LiCl pH 7.0, 30°C Arabidopsis thaliana

pI Value

Organism Comment pI Value Maximum pI Value
Arabidopsis thaliana calculated from sequence
-
 5.01