Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.3.95 extracted from

  • Bong, S.M.; Son, K.B.; Yang, S.W.; Park, J.W.; Cho, J.W.; Kim, K.T.; Kim, H.; Kim, S.J.; Kim, Y.J.; Lee, B.I.
    Crystal structure of human myotubularin-related protein 1 provides insight into the structural basis of substrate specificity (2016), PLoS ONE, 11, e0152611 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.95

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta 2(DE3) cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 100 mM Na-HEPES (pH 7.0) and 15% (w/v) polyethylene glycol 20000 Homo sapiens

Protein Variants

Protein Variants Comment Organism
C438S inactive Homo sapiens
D443A almost inactive Homo sapiens
K285A the mutation has little effect on the phosphatase activity compared to the wild type enzyme Homo sapiens
K484A the mutant exhibits no phosphatase activity Homo sapiens
R444A inactive Homo sapiens
R444A/R480A inactive Homo sapiens
R480A the mutant shows slightly reduced phosphatase activity compared to the wild type enzyme Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate + H2O Homo sapiens
-
 1-phosphatidyl-1D-myo-inositol 5-phosphate + phosphate
-
 ?
1-phosphatidyl-1D-myo-inositol 5-phosphate + H2O Homo sapiens
-
 1-phosphatidyl-1D-myo-inositol + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q13613
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate + H2O
-
 Homo sapiens 1-phosphatidyl-1D-myo-inositol 5-phosphate + phosphate
-
 ?
1-phosphatidyl-1D-myo-inositol 5-phosphate + H2O
-
 Homo sapiens 1-phosphatidyl-1D-myo-inositol + phosphate
-
 ?
di-C8 phosphatidylinositol 3,5-bisphosphate + H2O
-
 Homo sapiens ?
-
 ?
di-C8 phosphatidylinositol 5-phosphate + H2O
-
 Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
monomer dynamic light scattering Homo sapiens

Synonyms

Synonyms Comment Organism
MTMR1
-
 Homo sapiens
myotubularin-related protein 1
-
 Homo sapiens