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Literature summary for 3.1.3.85 extracted from
- Glucosylglycerate biosynthesis in the deepest lineage of the bacteria: characterization of the thermophilic proteins GpgS and GpgP from Persephonella marina (2007), J. Bacteriol., 189, 1648-1654.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpressed as His-tagged proteins in Escherichia coli | Persephonella marina |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Persephonella marina |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.19 | - |
2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | pH 7.0, 70°C | Persephonella marina | |
| 0.21 | - |
2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | pH 7.0, 90°C | Persephonella marina | |
| 0.36 | - |
2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | pH 7.0, 70°C | Persephonella marina | |
| 0.41 | - |
2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | pH 7.0, 90°C | Persephonella marina | |
| 0.43 | - |
2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | pH 7.0, 85°C | Persephonella marina | |
| 0.53 | - |
2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | pH 7.0, 85°C | Persephonella marina | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | stimulates activity with 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate or 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate | Persephonella marina | |
| Mg2+ | 0.1 mM is sufficient to promote the dephosphorylation of 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate. Maximal activation of dephosphorylation of 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate at 0.1 mM Mg2+. Maximal activation of dephosphorylation of 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate at 10 mM Mg2+ | Persephonella marina | |
| Mn2+ | stimulates activity with 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate or 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate | Persephonella marina | |
| additional information | K+, Ca2+, Sr2+, Cu2+, Ba2+, and Zn2+, do not stimulate GpgP activity at any concentration | Persephonella marina |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31200 | - |
1 * 31200, calculated from sequence | Persephonella marina |
| 39160 | - |
gel filtration | Persephonella marina |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate + H2O | Persephonella marina | in Persephonella marina two pathways for synthesis of glucosylglycerate are present: 1. the single-step pathway in with glucosylglycerate synthase (Ggs) catalyzes the synthesis of 2-O-(alpha-D-glucopyranosyl)-D-glycerate in one-step from ADP-glucose and D-glycerate, and 2. the two-step pathway in which glucosyl-3-phosphoglycerate synthase (GpgS) catalyzes the conversion of NDP-glucose and D-3-phosphoglycerate into glucosyl-3-phosphoglycerate, which is then converted to 2-O-(alpha-D-glucopyranosyl)-D-glycerate by glucosyl-3-phosphoglycerate phosphatase (GpgP) | 2-O-(alpha-D-glucosyl)-D-glycerate + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Persephonella marina | A9QXB5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant GpgS protein | Persephonella marina |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate + H2O | the Vmax/Km-value for 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate is 6-7fold higher than the value for 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate, depending on assay temperature | Persephonella marina | 2-O-(alpha-D-glucopyranosyl)-D-glycerate + phosphate | - |
? | |
| 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate + H2O | in Persephonella marina two pathways for synthesis of glucosylglycerate are present: 1. the single-step pathway in with glucosylglycerate synthase (Ggs) catalyzes the synthesis of 2-O-(alpha-D-glucopyranosyl)-D-glycerate in one-step from ADP-glucose and D-glycerate, and 2. the two-step pathway in which glucosyl-3-phosphoglycerate synthase (GpgS) catalyzes the conversion of NDP-glucose and D-3-phosphoglycerate into glucosyl-3-phosphoglycerate, which is then converted to 2-O-(alpha-D-glucopyranosyl)-D-glycerate by glucosyl-3-phosphoglycerate phosphatase (GpgP) | Persephonella marina | 2-O-(alpha-D-glucosyl)-D-glycerate + phosphate | - |
? | |
| 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate + H2O | the Vmax/Km-value for 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate is 6-7fold higher than the value for 2-O-(alpha-D-mannosyl)-3-phospho-D-glycerate, depending on assay temperature | Persephonella marina | 2-O-(alpha-D-mannopyranosyl)-D-glycerate + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 31200, calculated from sequence | Persephonella marina |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GpgP | - |
Persephonella marina |
| GpgP protein | - |
Persephonella marina |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
- |
Persephonella marina |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 100 | 60°C: about 30% of maximal activity with 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate as substrate, about 50% of maximal activity with 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate as substrate, 100°C: about 15% of maximal activity with 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate or 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate as substrate | Persephonella marina |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
half-life: 462 min | Persephonella marina |
| 85 | - |
half-life: 12 min | Persephonella marina |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate as substrate | Persephonella marina |
| 7 | - |
2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate as substrate | Persephonella marina |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.3 | 7.5 | pH 5.3: about 60% of maximal activity with 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate or 2-O-(alpha-D-mannopyranosyl)-3-phospho-D-glycerate as substrate, pH 8.5: about 35% of maximal activity with 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate as substrate, about 80% of maximal activity with 2-O-(alpha-D-mannosyl)-3-phospho-D-glycerate as substrate | Persephonella marina |
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