Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.36 | - |
myo-inositol hexakisphosphate | pH 7.4, 37°C, presence of Ca2+ | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required, assay in presence of 1.5 mM. The removal of Ca2+ causes a change in the CD-spectrum. The addition of Ca2+ to the apoenzyme results in an increase in the mean residue ellipticity, indicating a recovery of beta-sheet elements | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47500 | - |
x * 47500, SDS-PAGE | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | H9C9P0 | subsp. spizizenii | - |
Renatured (Comment) | Organism |
---|---|
metal-depleted apoenzyme can be restored by Ca2+, Sr2+,Co2+, Ba2+, Cu2+, La3+, Ni+, Tb2+, and Mn2+ to 44.1%, 29.8%, 36.7%, 26.9%, 1.9%, 30.0%, 34.2%, 8.3%, and 34.9% of its reference activity, respectively | Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
14 | - |
pH 7.4, 37°C, presence of Ca2+ | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
myo-inositol hexakisphosphate + H2O | - |
Bacillus subtilis | myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 47500, SDS-PAGE | Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
47.8 | - |
melting temperature | Bacillus subtilis |
62.4 | - |
melting temperature, presence of Ca2+ | Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
25.4 | - |
myo-inositol hexakisphosphate | pH 7.4, 37°C, presence of Ca2+ | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Bacillus subtilis |