Application | Comment | Organism |
---|---|---|
analysis | comparison of assay methods and the impact of assay conditions on activity estimate using the molybdenum blue method, the molybdovanadate method, and the acetone phosphomolybdate method. Nearly identical activity of PhyA is determined from the molybdenum blue method and the acetone phosphomolybdate method. The molybdenum blue method and the molybdovanadate method give only 22% difference in PhyA activity | Aspergillus niger |
analysis | comparison of assay methods and the impact of assay conditions on activity estimate using the molybdenum blue method, the molybdovanadate method, and the acetone phosphomolybdate method. The activity values of AppA2 are more variable with the three assay methods than those of Aspergillus niger PhyA. The molybdenum blue method and the molybdovanadate method produce nearly a 3fold disparity for AppA2. Overall, the pH value, the type of buffers, and the inclusion of ancillary chemicals such as the detergents Triton X-100 and BSA each account for approximately one-third of the variations of AppA2 | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Escherichia coli |
expression in Pichia pastoris | Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.034 | - |
myo-inositol hexakisphosphate | pH 3.5, 37°C | Aspergillus niger | |
0.074 | - |
myo-inositol hexakisphosphate | pH 3.5, 37°C | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
x * 47000, SDS-PAGE | Escherichia coli |
84000 | - |
x * 84000, SDS-PAGE | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Escherichia coli | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Escherichia coli |
glycoprotein | - |
Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
myo-inositol hexakisphosphate + H2O | - |
Escherichia coli | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
myo-inositol hexakisphosphate + H2O | - |
Aspergillus niger | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 47000, SDS-PAGE | Escherichia coli |
? | x * 84000, SDS-PAGE | Aspergillus niger |
Synonyms | Comment | Organism |
---|---|---|
AppA2 | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
170 | - |
myo-inositol hexakisphosphate | pH 3.5, 37°C | Aspergillus niger | |
840 | - |
myo-inositol hexakisphosphate | pH 3.5, 37°C | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5000 | - |
myo-inositol hexakisphosphate | pH 3.5, 37°C | Aspergillus niger | |
11400 | - |
myo-inositol hexakisphosphate | pH 3.5, 37°C | Escherichia coli |