Cloned (Comment) | Organism |
---|---|
arabinose-inducible expression of His-tagged wild-type and D9A mutant enzymes and N-terminal domains from cDNA in Escherichia coli strain BL21-AI, expression of strep-tagged enzyme from full-length gene in Escherichia coli strain XL1-Blue | Homo sapiens |
arabinose-inducible expression of the enzyme from full-length gene in Escherichia coli strain BL21-AI | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
D9A | site-directed mutagenesis, inactive mutant N-terminal domain | Homo sapiens |
additional information | the isolated recombinant N-terminal domain is a fully active phosphatase | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | complete inhibition at 20 mM | Homo sapiens | |
EDTA | complete inhibition at 20 mM | Mus musculus | |
EDTA | complete inhibition at 20 mM | Rattus norvegicus | |
additional information | no inhibition of the phosphatase activity by dicyclohexylurea and 4-fluorochalcone oxide | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Homo sapiens | |
additional information | - |
additional information | kinetics | Rattus norvegicus | |
0.24 | - |
4-nitrophenyl phosphate | pH 7.8, 37°C, native enzyme | Rattus norvegicus | |
0.37 | - |
4-nitrophenyl phosphate | pH 7.8, 37°C, recombinant enzyme | Rattus norvegicus | |
1.1 | - |
4-nitrophenyl phosphate | pH 7.8, 37°C, recombinant wild-type enzyme | Homo sapiens | |
2.3 | - |
4-nitrophenyl phosphate | pH 7.8, 37°C, recombinant wild-type N-terminal domain | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Mus musculus | 5829 | - |
cytosol | - |
Homo sapiens | 5829 | - |
cytosol | - |
Rattus norvegicus | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Mus musculus | |
Mg2+ | - |
Homo sapiens | |
Mg2+ | required for phosphatase activity | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mus musculus | - |
- |
- |
Rattus norvegicus | - |
male Fischer rats | - |
Purification (Comment) | Organism |
---|---|
from liver to homogeneity by ultracentrifugation and affinity chromatography on a benzyl thio resin using 4-fluorochalcone oxide as eluent | Rattus norvegicus |
recombinant His-tagged wild-type and D9A mutant enzymes and N-terminal domains from Escherichia coli strain BL21-AI by nickel affinity chromatography, recombinant strep-tagged wild-type enzyme from Escherichia coli strain XL1-Blue by streptavidin affinity chromatography | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(9S,10S)-10-hydroxy-9-(phosphooxy)octadecanoate + H2O = (9S,10S)-9,10-dihydroxyoctadecanoate + phosphate | the phosphatase activity of the enzyme is located at the N-terminal part, the C-terminal part harbors the epoxide hydrolase activity of EC 3.3.2.10, both catalytic sites act independently | Mus musculus | |
(9S,10S)-10-hydroxy-9-(phosphooxy)octadecanoate + H2O = (9S,10S)-9,10-dihydroxyoctadecanoate + phosphate | the phosphatase activity of the enzyme is located at the N-terminal part, the C-terminal part harbors the epoxide hydrolase activity of EC 3.3.2.10, both catalytic sites act independently | Homo sapiens | |
(9S,10S)-10-hydroxy-9-(phosphooxy)octadecanoate + H2O = (9S,10S)-9,10-dihydroxyoctadecanoate + phosphate | the phosphatase activity of the enzyme is located at the N-terminal part, the C-terminal part harbors the epoxide hydrolase activity of EC 3.3.2.10, both catalytic sites act independently | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Mus musculus | - |
liver | - |
Homo sapiens | - |
liver | - |
Rattus norvegicus | - |
Storage Stability | Organism |
---|---|
-70°C, 0.5 mg/ml purified enzyme, completely stable | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl phosphate + H2O | - |
Rattus norvegicus | 4-methylumbelliferone + phosphate | - |
? | |
4-nitrophenyl phosphate + H2O | - |
Mus musculus | 4-nitrophenol + phosphate | - |
? | |
4-nitrophenyl phosphate + H2O | - |
Homo sapiens | 4-nitrophenol + phosphate | - |
? | |
4-nitrophenyl phosphate + H2O | - |
Rattus norvegicus | 4-nitrophenol + phosphate | - |
? | |
additional information | no activity with 4-nitrophenyl sulfate of the isolated recombinant N-terminal domain | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Mus musculus |
dimer | - |
Homo sapiens |
dimer | - |
Rattus norvegicus |
More | the phosphatase activity of the enzyme is located at the 35 kDa N-terminal part which has a alpha/beta-fold, the 25 kDa C-terminal part, with a different hydrolase alpha/beta-fold, harbors the epoxide hydrolase activity of EC 3.3.2.10, structure analysis | Mus musculus |
More | the phosphatase activity of the enzyme is located at the 35 kDa N-terminal part which has a alpha/beta-fold, the 25 kDa C-terminal part, with a different hydrolase alpha/beta-fold, harbors the epoxide hydrolase activity of EC 3.3.2.10, structure analysis | Homo sapiens |
More | the phosphatase activity of the enzyme is located at the 35 kDa N-terminal part which has a alpha/beta-fold, the 25 kDa C-terminal part, with a different hydrolase alpha/beta-fold, harbors the epoxide hydrolase activity of EC 3.3.2.10, structure analysis | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
More | EC 3.1.3.76 belongs to the haloacid dehalogenase superfamily of enzymes, c.f. EC 3.3.2.10 | Homo sapiens |
More | EC 3.1.3.76 belongs to the haloacid dehalogenase superfamily of enzymes, see also EC 3.3.2.10 | Mus musculus |
More | EC 3.1.3.76 belongs to the haloacid dehalogenase superfamily of enzymes, see also EC 3.3.2.10 | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
37 | - |
assay at | Homo sapiens |
37 | - |
assay at | Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0061 | - |
4-nitrophenyl phosphate | pH 7.8, 37°C, recombinant wild-type enzyme | Homo sapiens | |
0.013 | - |
4-nitrophenyl phosphate | pH 7.8, 37°C, recombinant wild-type N-terminal domain | Homo sapiens | |
0.84 | - |
4-nitrophenyl phosphate | pH 7.8, 37°C, native enzyme | Rattus norvegicus | |
1.61 | - |
4-nitrophenyl phosphate | pH 7.8, 37°C, recombinant enzyme | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Mus musculus |
7.8 | - |
assay at | Homo sapiens |
7.8 | - |
assay at | Rattus norvegicus |