Cloned (Comment) | Organism |
---|---|
gene pchP, phylogenetic tree, regulation of pchP gene expression is under the control of GbdR and NtrC. PchP expression in Escherichia coli as an N-terminal fusion to intein or a histidine tag | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
additional information | site-directed mutagenesis of the aspartyl, D31, D33, and threonyl, T35, residues of motif I, of the seryl, S166, residue of motif II, and of the lysyl, K242, glycyl, G261, and aspartyl residues, D262, D265, and D267, of motif III | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Acetylcholine | - |
Pseudomonas aeruginosa | |
betaine | - |
Pseudomonas aeruginosa | |
choline | - |
Pseudomonas aeruginosa | |
additional information | the enzyme contains two sites for alkylammonium compounds, one of which is located in the catalytic site near the metal ion-phosphoester pocket, while the other one is located in an inhibitory site responsible for the binding of the alkylammonium moiety. Both sites are close to each other and interact through the residues 42E, 43E and 82YYY84 | Pseudomonas aeruginosa | |
phosphorylcholine | - |
Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | activates, Zn2+ and Cu2+ are better activators than Mg2+ at pH 5.0 | Pseudomonas aeruginosa | |
Mg2+ | activates, Zn2+ and Cu2+ are better activators than Mg2+ at pH 5.0 | Pseudomonas aeruginosa | |
Zn2+ | activates, Zn2+ and Cu2+ are better activators than Mg2+ at pH 5.0. Zn2+ induces a pH-dependent a conformational change in the active center, at pH 5.0, that is communicated to the inhibitory site, producing a compact or closed structure. However, at pH 7.4, this effect is not observed because to the hydrolysis of the [Zn2+L-12 L02(H2O)2] complex, which causes a change from octahedral to tetrahedral in the metal coordination geometry | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphocholine + H2O | Pseudomonas aeruginosa | - |
choline + phosphate | - |
? | |
phosphoethanolamine + H2O | Pseudomonas aeruginosa | - |
ethanolamine + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q9HTR2 | gene pchP, locus PA5292 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | D31 is phosphorylated during phosphoester hydrolysis | Pseudomonas aeruginosa |
Purification (Comment) | Organism |
---|---|
recombinant PchP from Escherichia coli, as an N-terminal fusion to intein or a histidine tag, in the folded state | Pseudomonas aeruginosa |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
phosphocholine + H2O = choline + phosphate | catalytic mechanism, overview | Pseudomonas aeruginosa | |
phosphoethanolamine + H2O = ethanolamine + phosphate | catalytic mechanism, overview | Pseudomonas aeruginosa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | growth on choline, betaine, dimethylglycine, or carnitine | Pseudomonas aeruginosa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | phosphorylcholine phosphatase catalyzes the hydrolysis of 4-nitrophenylphosphate | Pseudomonas aeruginosa | ? | - |
? | |
phosphocholine + H2O | - |
Pseudomonas aeruginosa | choline + phosphate | - |
? | |
phosphocholine + H2O | substrate docking assay and structure, the oxygen atom of the carboxyl group of D31 is involved in nucleophilic attack on the phosphorus atom of the substrate, the D33 residue is important for catalysis because it participates in the phosphorylation of D31, overview. D262 and D267 are the aspartyl residues involved in catalysis | Pseudomonas aeruginosa | choline + phosphate | - |
? | |
phosphoethanolamine + H2O | - |
Pseudomonas aeruginosa | ethanolamine + phosphate | - |
? | |
phosphoethanolamine + H2O | substrate docking assay and structure, the oxygen atom of the carboxyl group of D31 is involved in nucleophilic attack on the phosphorus atom of the substrate, the D33 residue is important for catalysis because it participates in the phosphorylation of D31, overview. D262 and D267 are the aspartyl residues involved in catalysis | Pseudomonas aeruginosa | ethanolamine + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the active enzyme is a dimer, enzyme structure molecular modeling and molecular dynamics, with substrate and inhibitor binding sites, overview | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
PChP | - |
Pseudomonas aeruginosa |
phosphorylcholine phosphatase | - |
Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 6 | - |
Pseudomonas aeruginosa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | optimal range, substrate phosphocholine | Pseudomonas aeruginosa |
Organism | Comment | Expression |
---|---|---|
Pseudomonas aeruginosa | the regulation of pchP gene expression is under the control of GbdR and NtrC | additional information |
Pseudomonas aeruginosa | dimethylglycine is likely the metabolite directly involved in the induction of PchP. Induction of pchP transcription by glycine betaine, a product of choline oxidation, via betaine aldehyde, mediated by GbdR, an AraC family transcription factor | up |
General Information | Comment | Organism |
---|---|---|
metabolism | the regulation of choline metabolism and consequently PchP synthesis may reflect an adaptive response of Pseudomonas aeruginosa to environmental conditions. Regulation of pchP gene expression, overview | Pseudomonas aeruginosa |