BRENDA - Enzyme Database
show all sequences of 3.1.3.68

Characterization of a phosphatase specific for 2-deoxyglucose-6-phosphate in a yeast mutant

Martin, M.; Heredia, C.F.; FEBS Lett. 83, 245-248 (1977)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
fructose 1-phosphate
-
Saccharomyces cerevisiae
phosphate
-
Saccharomyces cerevisiae
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
15
-
2-deoxy-D-glucose 6-phosphate
-
Saccharomyces cerevisiae
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Cd2+
can replace Mg2+ in activation, with less than 15% of the efficiency
Saccharomyces cerevisiae
Co2+
can replace Mg2+ in activation, with 65% of the efficiency
Saccharomyces cerevisiae
Mn2+
can replace Mg2+ in activation, with less than 15% of the efficiency
Saccharomyces cerevisiae
Ni2+
can replace Mg2+ in activation, with 35% of the efficiency
Saccharomyces cerevisiae
Zn2+
can replace Mg2+ in activation, with less than 15% of the efficiency
Saccharomyces cerevisiae
Organism
Organism
UniProt
Commentary
Textmining
Saccharomyces cerevisiae
-
; the enzyme is also present in wild type yeast, although its level is ten to twenty times lower than those found in the mutant
-
Saccharomyces cerevisiae 10a12-13
-
-
-
Saccharomyces cerevisiae PM-II
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Saccharomyces cerevisiae
Storage Stability
Storage Stability
Organism
-10°C, stable for several weeks
Saccharomyces cerevisiae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
Fructose 1-phosphate + H2O
with 80% of the activity with 2-deoxy-D-glucose 6-phosphate
25144
Saccharomyces cerevisiae
Fructose + phosphate
-
-
-
-
Fructose 1-phosphate + H2O
with 80% of the activity with 2-deoxy-D-glucose 6-phosphate
25144
Saccharomyces cerevisiae 10a12-13
Fructose + phosphate
-
-
-
-
Fructose 1-phosphate + H2O
with 80% of the activity with 2-deoxy-D-glucose 6-phosphate
25144
Saccharomyces cerevisiae PM-II
Fructose + phosphate
-
-
-
-
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
35
-
pH 7, 5 min, , 20% loss of activity
Saccharomyces cerevisiae
40
-
pH 7, nearly complete loss of activity
Saccharomyces cerevisiae
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
7
-
Saccharomyces cerevisiae
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5
8
50% of maximal activity at pH 5 and 8
Saccharomyces cerevisiae
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
fructose 1-phosphate
-
Saccharomyces cerevisiae
phosphate
-
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
15
-
2-deoxy-D-glucose 6-phosphate
-
Saccharomyces cerevisiae
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Cd2+
can replace Mg2+ in activation, with less than 15% of the efficiency
Saccharomyces cerevisiae
Co2+
can replace Mg2+ in activation, with 65% of the efficiency
Saccharomyces cerevisiae
Mn2+
can replace Mg2+ in activation, with less than 15% of the efficiency
Saccharomyces cerevisiae
Ni2+
can replace Mg2+ in activation, with 35% of the efficiency
Saccharomyces cerevisiae
Zn2+
can replace Mg2+ in activation, with less than 15% of the efficiency
Saccharomyces cerevisiae
Purification (Commentary) (protein specific)
Commentary
Organism
-
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Saccharomyces cerevisiae
Storage Stability (protein specific)
Storage Stability
Organism
-10°C, stable for several weeks
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
Fructose 1-phosphate + H2O
with 80% of the activity with 2-deoxy-D-glucose 6-phosphate
25144
Saccharomyces cerevisiae
Fructose + phosphate
-
-
-
-
Fructose 1-phosphate + H2O
with 80% of the activity with 2-deoxy-D-glucose 6-phosphate
25144
Saccharomyces cerevisiae 10a12-13
Fructose + phosphate
-
-
-
-
Fructose 1-phosphate + H2O
with 80% of the activity with 2-deoxy-D-glucose 6-phosphate
25144
Saccharomyces cerevisiae PM-II
Fructose + phosphate
-
-
-
-
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
35
-
pH 7, 5 min, , 20% loss of activity
Saccharomyces cerevisiae
40
-
pH 7, nearly complete loss of activity
Saccharomyces cerevisiae
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
7
-
Saccharomyces cerevisiae
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5
8
50% of maximal activity at pH 5 and 8
Saccharomyces cerevisiae
Other publictions for EC 3.1.3.68
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
750667
Izawati
Evaluation on the effectivene ...
Saccharomyces cerevisiae
Front. Plant Sci.
6
727
2015
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1
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2
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1
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1
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1
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677697
Toivari
Metabolic engineering of Sacch ...
Saccharomyces cerevisiae
Appl. Environ. Microbiol.
73
5471-5476
2007
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1
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1
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1
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1
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4
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2
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1
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1
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1
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1
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4
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1
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25141
Randez-Gil
DOGR1 and DOGR2: two genes fro ...
Saccharomyces cerevisiae
Yeast
11
1233-1240
1995
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1
2
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1
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1
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1
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8
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2
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1
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1
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2
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1
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1
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8
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2
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1
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25142
Randez-Gil
The expression of a specific 2 ...
Saccharomyces cerevisiae
Curr. Genet.
28
101-107
1995
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1
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1
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3
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1
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25143
Sanz
Molecular characterization of ...
Saccharomyces cerevisiae
Yeast
10
1195-1202
1994
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-
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1
1
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2
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1
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1
1
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1
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25144
Martin
Characterization of a phosphat ...
Saccharomyces cerevisiae 10a12-13, Saccharomyces cerevisiae, Saccharomyces cerevisiae PM-II
FEBS Lett.
83
245-248
1977
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2
1
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5
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3
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1
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1
1
3
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2
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1
1
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2
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1
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5
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1
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1
1
3
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2
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1
1
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