Cloned (Comment) | Organism |
---|---|
gene SPy_0039, recombinant expression of His-tagged enzyme Sp-PTP in Escherichia coli strain BL21(DE3) | Streptococcus pyogenes serotype M1 |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged enzyme, sitting drop vapor diffusion method, mixing of 0.001 ml of 20 mg/ml protein in 20 mM Tris-HCl, pH 7.5, 200 mM NaCl, 5 mM 2-mercaptoethanol, and 2 mM dithiothreitol, with 0.001 ml of precipitant solution containing 150 mM potassium thiocyanate, 100 mM Bis-Tris, pH 6.25, 8% PEG 3350, and 2% D-sorbitol, 18°C, X-ray diffraction structure determination and analysis at 1.9 A resolution, molecular replacement method using the structure of the YfkJ from Bacillus subtilis as a search model | Streptococcus pyogenes serotype M1 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[a protein]-tyrosine phosphate + H2O | Streptococcus pyogenes serotype M1 | - |
[a protein]-tyrosine + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pyogenes serotype M1 | Q9A1Y0 | a Group A Streptococcus | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme Sp-PTP from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Streptococcus pyogenes serotype M1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Streptococcus pyogenes serotype M1 | 4-nitrophenol + phosphate | - |
? | |
additional information | enzyme SP-PTP shares high similarity with typical low molecular weight protein tyrosine phosphatases (LMWPTPs), which are specific for phosphotyrosine, but not with dual-specificity phosphatases, in overall folding and active site composition. In the dephosphorylation activity test, SP-PTP consistently acts on phosphotyrosine substrates, but not or only minimally on phosphoserine/phosphothreonine substrates. PTP as a canonical tyrosine-specific LMWPTP | Streptococcus pyogenes serotype M1 | ? | - |
? | |
[a protein]-tyrosine phosphate + H2O | - |
Streptococcus pyogenes serotype M1 | [a protein]-tyrosine + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the SP-PTP molecule is composed of four parallel beta-strands [beta1(Lys3-Val7), beta2(Met32-Ala37), beta3(Tyr81-Gly84), and beta4(Ile105-Leu107)] sandwiched on both sides by five alpha-helices [a1(Cys13-Ile25), alpha2(Ser49-Tyr58), alpha3(Ile73-Thr78), alpha4(Ser87-Ser97), and alpha5(Phe122-Leu140)], which are arranged to form the Rossmann fold, which is typical of LMWPTPs | Streptococcus pyogenes serotype M1 |
Synonyms | Comment | Organism |
---|---|---|
LMWPTP | - |
Streptococcus pyogenes serotype M1 |
low molecular weight protein tyrosine phosphatase | - |
Streptococcus pyogenes serotype M1 |
SP-PTP | - |
Streptococcus pyogenes serotype M1 |
SPy_0039 | - |
Streptococcus pyogenes serotype M1 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Streptococcus pyogenes serotype M1 |
General Information | Comment | Organism |
---|---|---|
additional information | enzyme structure analysis and comparisons, overview | Streptococcus pyogenes serotype M1 |