Protein Variants | Comment | Organism |
---|---|---|
D445A | almost complete loss of activity | Rattus norvegicus |
D54A | almost complete loss of activity | Rattus norvegicus |
N49A | 38% of activity of wild type | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
29.3 | - |
p-nitrophenyl phosphate | pH 7.8, wild type enzyme | Rattus norvegicus | |
48.9 | - |
p-nitrophenyl phosphate | pH 7.8, D445A mutant | Rattus norvegicus | |
55.1 | - |
p-nitrophenyl phosphate | pH 7.8, N49A mutant | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required for binding of catalytic subunit | Rattus norvegicus | |
Mg2+ | required, Km-value about 0.3-0.5 mM | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
p-nitrophenyl phosphate + H2O | - |
Rattus norvegicus | p-nitrophenol + phosphate | - |
? |