Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.3.24 extracted from

  • Cumino, A.; Ekeroth, C.; Salerno, G.L.
    Sucrose-phosphate phosphatase from Anabaena sp. strain PCC 7120: isolation of the protein and gene revealed significant structural differences from the higher-plant enzyme (2001), Planta, 214, 250-256.
    View publication on PubMed
Search for more literature for 3.1.3.24

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, expression in Escherichia coli DH5alpha as His-tagged enzyme Anabaena sp.

Inhibitors

Inhibitors Comment Organism Structure
fluoride 5% inhibition at 2.5 mM Anabaena sp.
molybdate 12% inhibition at 2.5 mM Anabaena sp.
sucrose weak competitive product inhibition Anabaena sp.
vanadate 50% inhibition at 2.5 mM Anabaena sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.35
-
 sucrose 6-phosphate
-
 Anabaena sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Anabaena sp.
Mn2+
-
 Anabaena sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000 27000 native enzyme, gel filtration Anabaena sp.
28000
-
 1 * 28000, native enzyme, SDS-PAGE, 1 * 28034, native enzyme, amino acid sequence calculation, 1 * 32000, recombinant His-tagged enzyme, SDS-PAGE Anabaena sp.
28034
-
 1 * 28000, native enzyme, SDS-PAGE, 1 * 28034, native enzyme, amino acid sequence calculation, 1 * 32000, recombinant His-tagged enzyme, SDS-PAGE Anabaena sp.
32000
-
 1 * 28000, native enzyme, SDS-PAGE, 1 * 28034, native enzyme, amino acid sequence calculation, 1 * 32000, recombinant His-tagged enzyme, SDS-PAGE Anabaena sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sucrose 6-phosphate + H2O Anabaena sp.
-
 sucrose + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Anabaena sp. Q93M81 strain PCC 7120
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli partially, and native for strain PCC 7120 3260fold Anabaena sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15.66
-
 purified enzyme Anabaena sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with glucose 1-phosphate, fructose 6-phosphate, fructose 1,6-bisphosphate, glucose 1,6-bisphosphate, and trehalose 6-phosphate Anabaena sp. ?
-
 ?
sucrose 6-phosphate + H2O
-
 Anabaena sp. sucrose + phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 28000, native enzyme, SDS-PAGE, 1 * 28034, native enzyme, amino acid sequence calculation, 1 * 32000, recombinant His-tagged enzyme, SDS-PAGE Anabaena sp.

Synonyms

Synonyms Comment Organism
sucrose-phosphate phosphatase
-
 Anabaena sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Anabaena sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
80
-
 sucrose
-
 Anabaena sp.