Literature summary for 3.1.3.12 extracted from

Martinez-Esparza, M.; Martinez-Vicente, E.; Gonzalez-Parraga, P.; Ros, J.M.; Garcia-Penarrubia, P.; Argueelles, J.C.
Role of trehalose-6P phosphatase (TPS2) in stress tolerance and resistance to macrophage killing in Candida albicans (2009), Int. J. Med. Microbiol., 299, 453-464.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Candida albicans

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
trehalose 6-phosphate + H2O	Candida albicans	pleiotropic defective phenotype of disrupted TPS2, cell wall integrity and ability to form chlamydospores maintained, reduced growth and temperature sensitivity (42°C) of homozygous mutant, severe oxidative exposure (50 mM H2O2), null mutants reveal adaptive antioxidant response and cross-tolerance between temperature and oxidative stress, expression of TPS2 and TPS1 genes promoted in wild-type cells in response to acute (50 mM) but not gentle (5 mM) oxidative exposure	trehalose + phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Candida albicans	Q5AI14	wild-type SC5314 and mutant	-

Purification (Commentary)

Purification (Comment)	Organism
gel filtration	Candida albicans

Source Tissue

Source Tissue	Comment	Organism	Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	pleiotropic defective phenotype of disrupted TPS2, cell wall integrity and ability to form chlamydospores maintained, reduced growth and temperature sensitivity (42°C) of homozygous mutant, severe oxidative exposure (50 mM H2O2), null mutants reveal adaptive antioxidant response and cross-tolerance between temperature and oxidative stress, differential measurement of trehalose and trehalose 6-phosphate by HPLC, accumulation of trehalose 6-phosphate in null mutants but not in parental cells, accumulation of trehalose 6-phosphate enhanced after oxidative exposure, expression of TPS2 and TPS1 genes promoted in wild-type cells in response to acute (50 mM) but not gentle (5 mM) oxidative exposure, transcriptional response absent in mutants, co-culture of yeast cells with macrophages and fungicidal assays, role of trehalose biosynthetic pathway in the cellular response to oxidative stress	Candida albicans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
trehalose 6-phosphate + H2O	pleiotropic defective phenotype of disrupted TPS2, cell wall integrity and ability to form chlamydospores maintained, reduced growth and temperature sensitivity (42°C) of homozygous mutant, severe oxidative exposure (50 mM H2O2), null mutants reveal adaptive antioxidant response and cross-tolerance between temperature and oxidative stress, expression of TPS2 and TPS1 genes promoted in wild-type cells in response to acute (50 mM) but not gentle (5 mM) oxidative exposure	Candida albicans	trehalose + phosphate	-	?
trehalose 6-phosphate + H2O	protective role of the trehalose biosynthetic pathway in the cellular response to oxidative stress and subsequently in the resistance to phagocytosis	Candida albicans	trehalose + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
TPS2	-	Candida albicans

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Release 2023.1 (January 2023)