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Literature summary for 3.1.3.12 extracted from
- The C. elegans lethal gut-obstructed gob-1 gene is trehalose-6-phosphate phosphatase (2005), Dev. Biol., 287, 35-47.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the gene gob-1 is activates by transcription factor ELT-2, which is sufficient but not necessary in embryonic intestine, overview | Caenorhabditis elegans |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene gob-1, DNA and amino acid sequence determination and analysis, expression pattern, expression of GST-tagged enzyme in Escherichia coli strain BL21(DE3) | Caenorhabditis elegans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | 0.15 | trehalose 6-phosphate | pH 7.0, 22°C, recombinant enzyme | Caenorhabditis elegans |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Caenorhabditis elegans | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Caenorhabditis elegans | the gene gob-1, encoding the enzyme, loss of function causes the lethal gut-obstructed phenotype in the worms, analysis of phenotype including larval lethality, overview | ? | - |
? | |
| trehalose 6-phosphate + H2O | Caenorhabditis elegans | - |
trehalose + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Caenorhabditis elegans | - |
gene gob-1, several strains | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant GST-tagged enzyme from Escherichia coli strain BL21(DE3) | Caenorhabditis elegans |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| body wall muscle | - |
Caenorhabditis elegans | - |
| embryo | - |
Caenorhabditis elegans | - |
| gut | lethal gut-obstructed-gene 1, gob-1 | Caenorhabditis elegans | - |
| hypodermis | - |
Caenorhabditis elegans | - |
| intestine | - |
Caenorhabditis elegans | - |
| additional information | expression pattern of gene gob-1 during development | Caenorhabditis elegans | - |
| neuron | - |
Caenorhabditis elegans | - |
| pharynx | - |
Caenorhabditis elegans | - |
| tail | - |
Caenorhabditis elegans | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the gene gob-1, encoding the enzyme, loss of function causes the lethal gut-obstructed phenotype in the worms, analysis of phenotype including larval lethality, overview | Caenorhabditis elegans | ? | - |
? | |
| trehalose 6-phosphate + H2O | - |
Caenorhabditis elegans | trehalose + phosphate | - |
? | |
| trehalose 6-phosphate + H2O | high substrate specificity, overview | Caenorhabditis elegans | trehalose + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GOB-1 | - |
Caenorhabditis elegans |
| More | the enzyme belongs to the HAD-like hydrolase superfamily | Caenorhabditis elegans |
| trehalose-6-phosphate phosphatase | - |
Caenorhabditis elegans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at room temperature | Caenorhabditis elegans |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8 | - |
trehalose 6-phosphate | pH 7.0, 22°C, recombinant enzyme | Caenorhabditis elegans | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
neutral pH optimum | Caenorhabditis elegans |
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