BRENDA - Enzyme Database
show all sequences of 3.1.3.12

Interactions among sites responsible for trehalose 6-phosphate and trehalose-activated glucose 6-phosphate hydrolysis on trehalose phosphatase isolated from Phormia regina

Friedman, S.; J. Biol. Chem. 246, 4122-4130 (1971)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
trehalose
activates hydrolysis of glucose 6-phosphate, no activation of trehalose 6-phosphate
Phormia regina
Inhibitors
Inhibitors
Commentary
Organism
Structure
glucose
competitive inhibition of hydrolysis of glucose 6-phosphate, ineffective against hydrolysis of trehalose 6-phosphate
Phormia regina
phosphate
-
Phormia regina
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.25
0.5
glucose 6-phosphate
-
Phormia regina
0.4
0.7
trehalose 6-phosphate
-
Phormia regina
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
required, optimal concentration: 4 mM
Phormia regina
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25000
26000
gel filtration, sucrose density gradient centrifugation
Phormia regina
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Phormia regina
-
-
-
Purification (Commentary)
Commentary
Organism
-
Phormia regina
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.631
-
trehalose activated hydrolysis of glucose 6-phosphate
Phormia regina
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-Deoxy-D-glucose 6-phosphate + H2O
-
134587
Phormia regina
2-Deoxy-D-glucose + phosphate
-
-
-
?
D-fructose 6-phosphate + H2O
-
134587
Phormia regina
D-fructose + phosphate
-
-
-
?
glucose 6-phosphate + H2O
activated by trehalose
134587
Phormia regina
?
-
-
-
?
trehalose-6-phosphate + H2O
-
134587
Phormia regina
trehalose + phosphate
-
134587
Phormia regina
?
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
53
-
5 min, 25% loss of activity
Phormia regina
60
-
5 min, 75-90% loss of activity
Phormia regina
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
6.5
hydrolysis of trehalose 6-phosphate
Phormia regina
6
-
hydrolysis of glucose 6-phosphate in presence of trehalose
Phormia regina
7
-
hydrolysis of glucose 6-phosphate in absence of trehalose
Phormia regina
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
trehalose
activates hydrolysis of glucose 6-phosphate, no activation of trehalose 6-phosphate
Phormia regina
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
glucose
competitive inhibition of hydrolysis of glucose 6-phosphate, ineffective against hydrolysis of trehalose 6-phosphate
Phormia regina
phosphate
-
Phormia regina
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.25
0.5
glucose 6-phosphate
-
Phormia regina
0.4
0.7
trehalose 6-phosphate
-
Phormia regina
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
required, optimal concentration: 4 mM
Phormia regina
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25000
26000
gel filtration, sucrose density gradient centrifugation
Phormia regina
Purification (Commentary) (protein specific)
Commentary
Organism
-
Phormia regina
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.631
-
trehalose activated hydrolysis of glucose 6-phosphate
Phormia regina
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-Deoxy-D-glucose 6-phosphate + H2O
-
134587
Phormia regina
2-Deoxy-D-glucose + phosphate
-
-
-
?
D-fructose 6-phosphate + H2O
-
134587
Phormia regina
D-fructose + phosphate
-
-
-
?
glucose 6-phosphate + H2O
activated by trehalose
134587
Phormia regina
?
-
-
-
?
trehalose-6-phosphate + H2O
-
134587
Phormia regina
trehalose + phosphate
-
134587
Phormia regina
?
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
53
-
5 min, 25% loss of activity
Phormia regina
60
-
5 min, 75-90% loss of activity
Phormia regina
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
6.5
hydrolysis of trehalose 6-phosphate
Phormia regina
6
-
hydrolysis of glucose 6-phosphate in presence of trehalose
Phormia regina
7
-
hydrolysis of glucose 6-phosphate in absence of trehalose
Phormia regina
Other publictions for EC 3.1.3.12
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
750320
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12
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-
-
-
5
-
3
-
-
-
3
-
-
-
-
-
12
-
-
-
-
-
-
-
12
15
-
3
-
-
-
5
-
-
-
-
-
-
-
-
5
-
3
-
-
-
3
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4
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-
-
2
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1
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-
-
-
-
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2
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4
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-
-
2
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4
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2
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1
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1
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1
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750508
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1
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1
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-
-
1
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-
-
-
-
-
-
-
1
-
-
4
-
-
1
-
-
-
-
-
1
-
-
-
1
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-
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1
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1
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1
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2
2
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752217
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5
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10
5
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6
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10
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5
-
-
-
-
12
-
5
-
-
-
5
-
-
-
-
-
-
5
-
-
-
-
-
-
-
10
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5
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-
-
6
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-
-
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12
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1
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2
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6
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-
1
-
-
-
-
-
4
2
1
-
-
-
1
-
-
-
-
-
-
-
1
1
-
1
7
-
-
-
-
-
-
1
-
2
-
-
-
1
-
-
-
-
4
2
1
-
-
-
1
-
-
-
-
3
3
-
-
-
750627
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2
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-
-
-
-
-
2
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-
-
-
-
-
-
-
-
-
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-
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-
1
-
-
1
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2
-
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-
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2
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1
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-
-
-
-
-
2
-
5
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
5
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
7
7
-
-
-
751641
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1
-
1
-
-
-
-
-
-
1
-
4
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
751680
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15124
2015
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-
1
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
1
4
4
1
-
-
728980
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-
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1
-
-
1
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
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-
-
-
1
-
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-
-
-
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1
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-
1
-
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-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
729783
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Front. Plant Sci.
5
418
2014
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
-
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-
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1
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1
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-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
729789
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63
24-41
2014
-
-
-
-
-
-
-
-
-
-
-
2
-
9
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
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-
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-
-
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-
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-
2
-
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-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
730106
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229
1245-1255
2014
-
-
-
-
-
-
3
2
-
3
2
1
-
4
-
-
1
-
-
-
2
-
2
1
1
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
3
-
2
-
3
2
1
-
-
-
1
-
-
2
-
2
1
1
-
1
-
1
-
1
-
1
-
-
1
-
-
730793
Farelli
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PLoS Pathog.
10
e1004245
2014
-
-
1
1
16
-
3
13
-
1
-
1
-
7
-
-
1
-
-
-
-
-
1
-
-
-
-
15
-
-
-
-
3
-
-
-
-
1
-
1
16
-
-
3
3
13
-
1
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
15
-
-
-
-
-
-
-
-
15
15
728601
Zaparty
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PLoS One
8
e61354
2013
-
-
1
-
-
-
-
-
-
-
3
-
-
9
-
-
-
-
-
-
1
-
9
1
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
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3
-
-
-
-
-
-
-
1
-
9
1
1
-
-
-
-
-
-
-
-
1
1
-
-
-
728859
Shi
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Acta Biochim. Biophys. Sin.
45
837-844
2013
-
-
1
-
-
-
-
-
-
1
2
2
-
5
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
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-
1
-
-
-
-
-
-
-
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-
1
2
2
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
730652
Van Houtte
Redundant and non-redundant ro ...
Arabidopsis thaliana
Plant Signal. Behav.
8
e23209
2013
-
-
-
-
-
-
-
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-
-
2
-
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3
-
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3
-
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-
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
730665
Bargaz
Differential expression of tre ...
Phaseolus vulgaris
Planta
238
107-119
2013
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
2
-
-
1
-
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1
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2
-
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1
-
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-
-
-
-
-
-
1
1
1
1
-
-
730747
Kushwaha
Immunization of Mastomys couch ...
Brugia malayi
PLoS ONE
8
e72585
2013
-
1
1
-
-
-
-
-
-
-
-
1
-
5
-
-
1
-
-
-
-
-
1
-
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1
1
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1
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1
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1
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-
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-
-
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-
731023
Li
Cloning, expression and charac ...
Arthrobacter sp., Arthrobacter sp. A3
World J. Microbiol. Biotechnol.
28
2713-2721
2012
-
-
1
-
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Role of trehalose-6P phosphata ...
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Extensive expression regulatio ...
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Trehalose-6-phosphate synthase ...
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Overexpression of the trehalos ...
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695123
Chung
A trehalose 6-phosphate syntha ...
Callinectes sapidus
Saline Syst.
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Trehalose biosynthesis in resp ...
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675853
Cardoso
Biochemical and genetic charac ...
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Microbiology
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Schultz
The solubility of recombinant ...
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Shima
Biochemical characterization o ...
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679880
Zhang
Trehalose-6-phosphate phosphat ...
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Lopez
Increased trehalose biosynthes ...
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New Phytol.
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Ocon
Trehalose turnover during abio ...
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682472
Miranda
A bifunctional TPS-TPP enzyme ...
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Planta
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Petzold
Characterization and regulatio ...
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682725
Rao
Crystal structure of trehalose ...
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Crystal structure of trehalos ...
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Protein Sci.
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658670
Silva
Trehalose biosynthesis in Ther ...
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Extremophiles
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29-36
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Kormish
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Pramanik
Functional identification of a ...
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649392
Padilla
Overproduction of trehalose: h ...
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Edavana
Cloning and expression of the ...
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