Any feedback?
Literature summary for 3.1.3.12 extracted from
- Interactions among sites responsible for trehalose 6-phosphate and trehalose-activated glucose 6-phosphate hydrolysis on trehalose phosphatase isolated from Phormia regina (1971), J. Biol. Chem., 246, 4122-4130.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| trehalose | activates hydrolysis of glucose 6-phosphate, no activation of trehalose 6-phosphate | Phormia regina |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| glucose | competitive inhibition of hydrolysis of glucose 6-phosphate, ineffective against hydrolysis of trehalose 6-phosphate | Phormia regina | |
| phosphate | - |
Phormia regina | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.25 | 0.5 | glucose 6-phosphate | - |
Phormia regina | |
| 0.4 | 0.7 | trehalose 6-phosphate | - |
Phormia regina | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required, optimal concentration: 4 mM | Phormia regina | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25000 | 26000 | gel filtration, sucrose density gradient centrifugation | Phormia regina |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phormia regina | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Phormia regina |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.631 | - |
trehalose activated hydrolysis of glucose 6-phosphate | Phormia regina |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-Deoxy-D-glucose 6-phosphate + H2O | - |
Phormia regina | 2-Deoxy-D-glucose + phosphate | - |
? | |
| D-fructose 6-phosphate + H2O | - |
Phormia regina | D-fructose + phosphate | - |
? | |
| glucose 6-phosphate + H2O | activated by trehalose | Phormia regina | ? | - |
? | |
| trehalose-6-phosphate + H2O | - |
Phormia regina | trehalose + phosphate | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 53 | - |
5 min, 25% loss of activity | Phormia regina |
| 60 | - |
5 min, 75-90% loss of activity | Phormia regina |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 6.5 | hydrolysis of trehalose 6-phosphate | Phormia regina |
| 6 | - |
hydrolysis of glucose 6-phosphate in presence of trehalose | Phormia regina |
| 7 | - |
hydrolysis of glucose 6-phosphate in absence of trehalose | Phormia regina |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
