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Literature summary for 3.1.3.11 extracted from
- Overexpression of Calvin cycle enzyme fructose 1,6-bisphosphatase in Chlamydomonas reinhardtii has a detrimental effect on growth (2016), Algal Res., 14, 116-126 .
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene FBP1, recombinant expression of the FLAG-tagged plastidic enzyme from chloroplast expression vector pWD2-CpFBP1, that includes the Chlamydomonas reinhardtii FBP1 coding region synthesized with Chlamydomonas reinhardtii chloroplast codon-bias, flanked with psbD and psbA 5' and 3' regulatory sequences, respectively, in Chlamydomonas reinhardtii strain CC125 cells. Integration of insert from expression vector pWD2-CpFBP1 into the Chlamydomonas reinhardtii chloroplast genome. Under photoautotrophic growth conditions and/or with elevated CO2 levels, this 40% elevation in FBPase activity has a negative effect overall on growth rate and biomass production | Chlamydomonas reinhardtii |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Chlamydomonas reinhardtii | 9507 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Chlamydomonas reinhardtii |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose 1,6-bisphosphate + H2O | Chlamydomonas reinhardtii | - |
D-fructose 6-phosphate + phosphate | - |
? | |
| D-fructose 1,6-bisphosphate + H2O | Chlamydomonas reinhardtii CC125 | - |
D-fructose 6-phosphate + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chlamydomonas reinhardtii | A8IKQ0 | - |
- |
| Chlamydomonas reinhardtii CC125 | A8IKQ0 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose 1,6-bisphosphate + H2O | - |
Chlamydomonas reinhardtii | D-fructose 6-phosphate + phosphate | - |
? | |
| D-fructose 1,6-bisphosphate + H2O | - |
Chlamydomonas reinhardtii CC125 | D-fructose 6-phosphate + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45000, recombinant FLAG-tagged enzyme CpFBP1, SDS-PAGE | Chlamydomonas reinhardtii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CpFBP1 | - |
Chlamydomonas reinhardtii |
| CrFBPase | - |
Chlamydomonas reinhardtii |
| cyanobacterial fructose-1,6-bisphosphatase | - |
Chlamydomonas reinhardtii |
| cyanoFBPase-II | - |
Chlamydomonas reinhardtii |
| FBP1 | gene name, nuclear gene that encodes CrFBPase | Chlamydomonas reinhardtii |
| fructose 1,6-bisphosphatase | - |
Chlamydomonas reinhardtii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Chlamydomonas reinhardtii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.2 | - |
assay at | Chlamydomonas reinhardtii |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | Calvin cycle enzyme | Chlamydomonas reinhardtii |
| metabolism | the enzyme fructose 1,6-bisphosphatase takes part in the Calvin cycle, but unlike the situation in higher plants, FBPase probably does not catalyze a rate-limiting step in the Chlamydomonas reinhardtii Calvin cycle, since under photoautotrophic growth conditions and/or with elevated CO2 levels, an elevation in FBPase activity has a negative effect overall on growth rate and biomass production | Chlamydomonas reinhardtii |
| physiological function | overexpression of fructose 1,6-bisphosphatase has a detrimental effect on growth | Chlamydomonas reinhardtii |
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