Protein Variants | Comment | Organism |
---|---|---|
D14A | less than 10% of wild-type activity | Geobacillus stearothermophilus |
D56A | less than 10% of wild-type activity | Geobacillus stearothermophilus |
D58A | less than 10% of wild-type activity | Geobacillus stearothermophilus |
E10A | less than 10% of wild-type activity | Geobacillus stearothermophilus |
G11A | less than 10% of wild-type activity | Geobacillus stearothermophilus |
G31A | less than 10% of wild-type activity | Geobacillus stearothermophilus |
G61A | less than 10% of wild-type activity | Geobacillus stearothermophilus |
I52A | 60% of wild-type activity | Geobacillus stearothermophilus |
I64A | as active as wild-type enzyme | Geobacillus stearothermophilus |
L107A | less than 10% of wild-type activity | Geobacillus stearothermophilus |
L162A | about 25% of wild-type activity | Geobacillus stearothermophilus |
R65A | about 10% of wild-type activity | Geobacillus stearothermophilus |
Y152A | as active as wild-type enzyme | Geobacillus stearothermophilus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5S precursor rRNA + H2O | Geobacillus stearothermophilus | 5S rRNA maturation | mature 5S rRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
RNase M5 mutant enzymes | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5S precursor rRNA + H2O | - |
Geobacillus stearothermophilus | mature 5S rRNA | - |
? | |
5S precursor rRNA + H2O | 5S rRNA maturation | Geobacillus stearothermophilus | mature 5S rRNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ribonuclease M5 | - |
Geobacillus stearothermophilus |