Cloned (Comment) | Organism |
---|---|
gene At1g55140, sequence comparison and phylogenetic analysis | Arabidopsis thaliana |
gene At3g13740, sequence comparison and phylogenetic analysis | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of null mutants for both genes RNC3 and RNC4 from the T-DNA insertion collection, termed rnc3-1 and rnc4-1, respectively, and of a double knockout mutant rnc3/4. Phenotypes, overview. rRNA deficiencies observed in rnc3/4 can be complemented by an RNC transgene | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Arabidopsis thaliana | 9507 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q6NQK8 | - |
- |
Arabidopsis thaliana | Q94F12 | - |
- |
Synonyms | Comment | Organism |
---|---|---|
At1g55140 | locus name | Arabidopsis thaliana |
At3g13740 | locus name | Arabidopsis thaliana |
RNase III | - |
Arabidopsis thaliana |
RNC3 | - |
Arabidopsis thaliana |
RNC4 | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
evolution | the two Arabidopsis thaliana chloroplast Mini-RNase III-like enzymes share 75% amino acid sequence identity | Arabidopsis thaliana |
malfunction | whereas rnc3 and rnc4 null mutants have no visible phenotype, rnc3/rnc4 (rnc3/4) double mutants are slightly smaller and chlorotic compared with the wild-type. Imprecise maturation of 23S rRNA is observed in the rnc3/4 double mutant, suggesting that exoribonucleases generate staggered ends in the absence of specific Mini-III-catalyzed cleavages. A similar phenotype is found at the 3' end of the 16S rRNA, and the primary 4.5S rRNA transcript contained 3' extensions, suggesting that Mini-III catalyzes several processing events of the polycistronic rRNA precursor. The rnc3/4 mutant shows overaccumulation of a noncoding RNA complementary to the 4.5S-5S rRNA intergenic region, and its presence correlates with that of the extended 4.5S rRNA precursor. Mature 23S rRNA 3' ends are unaffected by mini-RNase III deficiency | Arabidopsis thaliana |
metabolism | the enzyme is involved in plastid rRNA maturation | Arabidopsis thaliana |
physiological function | RNase III proteins recognize double-stranded RNA structures and catalyze endoribonucleolytic cleavages that often regulate gene expression. Arabidopsis thaliana chloroplast mini-ribonuclease III participates in rRNA maturation and intron recycling, overview. Key role of Mini-III in intron and noncoding RNA regulation | Arabidopsis thaliana |