Protein Variants | Comment | Organism |
---|---|---|
C280E | RNase H activity similar to wild-type | Human immunodeficiency virus 1 |
C280H | RNase H activity similar to wild-type | Human immunodeficiency virus 1 |
C280L | RNase H activity similar to wild-type | Human immunodeficiency virus 1 |
C280M | RNase H activity similar to wild-type | Human immunodeficiency virus 1 |
C280P | significant reduction in RNase H activity. A heterodimer p66C280p/p51C280P shows about 8% of wild-type RNaseH activity, 6% of strand transfer activity, and 105% of DNA polymerase activity. A heterodimer p66C280P/p51 shows about 60% of wild-type RNaseH activity, 80% of strand transfer activity, and 100% of DNA polymerase activity. A heterodimer p66/p51C280W shows about 30% of wild-type RNaseH activity, 6% of strand transfer activity, and 99% of DNA polymerase activity | Human immunodeficiency virus 1 |
C280Q | RNase H activity similar to wild-type | Human immunodeficiency virus 1 |
C280R | RNase H activity similar to wild-type | Human immunodeficiency virus 1 |
C280S | RNase H activity similar to wild-type | Human immunodeficiency virus 1 |
C280W | significant reduction in RNase H activity. A heterodimer p66C280W/p51C280W shows about 11% of wild-type RNaseH activity, 6% of strand transfer activity, and 100% of DNA polymerase activity. A heterodimer p66C280W/p51 shows about 44% of wild-type RNaseH activity, 80% of strand transfer activity, and 98% of DNA polymerase activity. A heterodimer p66/p51C280W shows about 29% of wild-type RNaseH activity, 7% of strand transfer activity, and 100% of DNA polymerase activity | Human immunodeficiency virus 1 |
C280Y | RNase H activity similar to wild-type | Human immunodeficiency virus 1 |
additional information | chemical modifications by thiol-specific reagents of cysteine 280, located in a helix I in the thumb subdomain of the polymerase domain, affect substantially only the RNase H activity | Human immunodeficiency virus 1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
poly(rA)n-poly(dT)n | 37°C, wild-type p66/p51 | Human immunodeficiency virus 1 | |
0.0177 | - |
poly(rA)n-poly(dT)n | 37°C, mutant p66C280P/p51 | Human immunodeficiency virus 1 | |
0.0257 | - |
poly(rA)n-poly(dT)n | 37°C, mutant p66/p51C280P | Human immunodeficiency virus 1 | |
0.0318 | - |
poly(rA)n-poly(dT)n | 37°C, mutant p66C280W/p51 | Human immunodeficiency virus 1 | |
0.0383 | - |
poly(rA)n-poly(dT)n | 37°C, mutant p66/p51C280W | Human immunodeficiency virus 1 | |
0.0553 | - |
poly(rA)n-poly(dT)n | 37°C, mutant p66C280P/p51C280P | Human immunodeficiency virus 1 | |
0.0663 | - |
poly(rA)n-poly(dT)n | 37°C, mutant p66C280W/p51C280W | Human immunodeficiency virus 1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Human immunodeficiency virus 1 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-end-labeled 267 nt-long RNA annealed to 20 nt-long synthetic DNA + H2O | - |
Human immunodeficiency virus 1 | ? | - |
? | |
poly(rA)n-poly(dT)n + H2O | - |
Human immunodeficiency virus 1 | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
poly(rA)n-poly(dT)n | 37°C, mutant p66/p51C280P | Human immunodeficiency virus 1 | |
0.024 | - |
poly(rA)n-poly(dT)n | 37°C, mutant p66/p51C280W | Human immunodeficiency virus 1 | |
0.025 | - |
poly(rA)n-poly(dT)n | 37°C, mutant p66C280P/p51 | Human immunodeficiency virus 1 | |
0.029 | - |
poly(rA)n-poly(dT)n | 37°C, mutant p66C280P/p51C280P | Human immunodeficiency virus 1 | |
0.029 | - |
poly(rA)n-poly(dT)n | 37°C, mutant p66C280W/p51 | Human immunodeficiency virus 1 | |
0.031 | - |
poly(rA)n-poly(dT)n | 37°C, wild-type p66/p51 | Human immunodeficiency virus 1 | |
0.034 | - |
poly(rA)n-poly(dT)n | 37°C, mutant p66C280W/p51C280W | Human immunodeficiency virus 1 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
510 | - |
poly(rA)n-poly(dT)n | 37°C, mutant p66C280W/p51C280W | Human immunodeficiency virus 1 | |
520 | - |
poly(rA)n-poly(dT)n | 37°C, mutant p66C280P/p51C280P | Human immunodeficiency virus 1 | |
620 | - |
poly(rA)n-poly(dT)n | 37°C, mutant p66/p51C280W | Human immunodeficiency virus 1 | |
910 | - |
poly(rA)n-poly(dT)n | 37°C, mutant p66C280W/p51 | Human immunodeficiency virus 1 | |
930 | - |
poly(rA)n-poly(dT)n | 37°C, mutant p66/p51C280P | Human immunodeficiency virus 1 | |
1460 | - |
poly(rA)n-poly(dT)n | 37°C, mutant p66C280P/p51 | Human immunodeficiency virus 1 | |
10300 | - |
poly(rA)n-poly(dT)n | 37°C, wild-type p66/p51 | Human immunodeficiency virus 1 |