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Literature summary for 3.1.22.2 extracted from

  • Kato, M.; Ikeda, Y.
    On the deoxyribonucleases, K1 and K2, isolated from mycelia of Aspergillus oryzae. I. Isolation and purification of DNases K1 and K2 (1968), J. Biochem., 64, 321-328.
    View publication on PubMed
Search for more literature for 3.1.22.2

General Stability

General Stability Organism
inactivation up to 45øC Aspergillus oryzae

Inhibitors

Inhibitors Comment Organism Structure
EDTA 90% inhibition at 0.001 M Aspergillus oryzae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 30% activation at 0.02 M and 10% activation at 0.001 M Aspergillus oryzae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA + H2O Aspergillus oryzae
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
2fold fractionation on DEAE-cellulose with NaCL gradient elution, gel filtration on sephadex G-50 Aspergillus oryzae

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Aspergillus oryzae
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 49.7 U, reducing absorbance at 260 nm/h/mg DNA digested Aspergillus oryzae

Storage Stability

Storage Stability Organism
-5°C in polyethylen glycol or glycerol for 3 weeks Aspergillus oryzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O
-
 Aspergillus oryzae 3'-phosphopolynucleotide + 3'-phosphooligonucleotide
-
 ?
DNA + H2O
-
 Aspergillus oryzae ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 9.5
-
 Aspergillus oryzae