Organism | UniProt | Comment | Textmining |
---|---|---|---|
prophage P1 | - |
enzyme EcoP1I | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates | mechanism | prophage P1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | cleavage generally depends on ratio of enzyme to cleavage sites, in presence of S-adenosyl-L-methionine, substrates with two cleavage sites in inverted repeats are susceptible, in absence of S-adenosyl-L-methionine, presence of Na+ leads to cleavage only in substrates with two sites in inverted repeats, presence of K+ leads to cleavage of all substrates | prophage P1 | ? | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | allosteric activator and specificity factor that ensures that cleavage only occurs when two endonucleases bind two recognition sites in a designated orientation | prophage P1 |