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Literature summary for 3.1.21.4 extracted from

  • Tamulaitis, G.; Rutkauskas, M.; Zaremba, M.; Grazulis, S.; Tamulaitiene, G.; Siksnys, V.
    Functional significance of protein assemblies predicted by the crystal structure of the restriction endonuclease BsaWI (2015), Nucleic Acids Res., 43, 8100-8110.
    View publication on PubMed
Search for more literature for 3.1.21.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Geobacillus stearothermophilus

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method at 19°C Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus Q6UQ65
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O the enzyme recognizes a degenerated sequence 5'-W/CCGGW-3' (W stands for A or T and / denotes the cleavage site). It belongs to a large family of restriction enzymes that contain a conserved CCGG tetranucleotide in their target sites. It requires binding of two target sites for the optimal catalytic activity Geobacillus stearothermophilus ?
-
 ?

Subunits

Subunits Comment Organism
dimer in solution the BsaWI protein exists in a dimer-tetramer-oligomer equilibrium, but in the presence of specific DNA forms a tetramer bound to two target sites. Site-directed mutagenesis and kinetic experiments show that BsaWI is active as a tetramer and requires two target sites for optimal activity Geobacillus stearothermophilus
tetramer in solution the BsaWI protein exists in a dimer-tetramer-oligomer equilibrium, but in the presence of specific DNA forms a tetramer bound to two target sites. Site-directed mutagenesis and kinetic experiments show that BsaWI is active as a tetramer and requires two target sites for optimal activity Geobacillus stearothermophilus

Synonyms

Synonyms Comment Organism
BsaWI
-
 Geobacillus stearothermophilus
type IIF restriction endonuclease
-
 Geobacillus stearothermophilus