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Literature summary for 3.1.21.4 extracted from

  • Saravanan, M.; Bujnicki, J.M.; Cymerman, I.A.; Rao, D.N.; Nagaraja, V.
    Type II restriction endonuclease R.KpnI is a member of the HNH nuclease superfamily (2004), Nucleic Acids Res., 32, 6129-6135.
    View publication on PubMed
Search for more literature for 3.1.21.4

Protein Variants

Protein Variants Comment Organism
D148A only traces of DNA cleavage activity when used in large excess Klebsiella pneumoniae
D148G only traces of DNA cleavage activity when used in large excess Klebsiella pneumoniae
D148G binds DNA with 2times reduced affinity compared to wild-type protein Klebsiella pneumoniae
H149L no cleavage activity Klebsiella pneumoniae
H149L binds DNA with 10times reduced affinity compared to wild-type protein Klebsiella pneumoniae
Q115E deprotonated mutant EcoRI is defective in DNA binding at neutral pH Klebsiella pneumoniae
Q175E only traces of DNA cleavage activity when used in large excess Klebsiella pneumoniae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Klebsiella pneumoniae

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O R.KpnI cleaves the DNA sequence 5'-GGTAC-/-C-3', generating 3' four base overhangs Klebsiella pneumoniae double-stranded DNA fragments with terminal 5'-phosphates
-
 ?

Synonyms

Synonyms Comment Organism
R.KpnI
-
 Klebsiella pneumoniae