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Literature summary for 3.1.21.4 extracted from
- Two new thermostable type II restriction endonucleases from Thermus aquaticus: TatI and TauI, which recognize the novel nucleotide sequences 5'-W|GTACW-3' and 5'-GCSG|C-3' respectively (2001), FEMS Microbiol. Lett., 204, 253-257.
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermus aquaticus | - |
enzymes TatI, TauI | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Thermus aquaticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | TatI recognition site is 5-AGTACA-3, the enzyme cleaves between first and second nucleotides generating 5-ends protruding four bases, TauI recognition site is 5-GCGSGC-3, the enzyme cleaves between fourth and fifth nucleotides generating 3-ends protruding three bases | Thermus aquaticus | ? | - |
? |  |
| phage lambda DNA | - |
Thermus aquaticus | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
assay | Thermus aquaticus |
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