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Literature summary for 3.1.2.12 extracted from

  • Kato, N.; Sakazawa, C.; Nishizawa, T.; Tani, Y.; Yamada, H.
    Purificaton and characterization of S-formylglutathione hydrolase from a methanol-utilizing yeast, Kloeckera sp. No. 2201 (1980), Biochim. Biophys. Acta, 611, 323-332.
    View publication on PubMed
Search for more literature for 3.1.2.12

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoate)
-
 Kloeckera sp.
arsenite
-
 Kloeckera sp.
CuSO4
-
 Kloeckera sp.
FeSO4
-
 Kloeckera sp.
HgCl2
-
 Kloeckera sp.
iodoacetate
-
 Kloeckera sp.
N-ethylmaleimide
-
 Kloeckera sp.
p-chloromercuribenzoate
-
 Kloeckera sp.
p-hydroxymercuribenzoate
-
 Kloeckera sp.
Zn2+
-
 Kloeckera sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.077
-
 S-formylglutathione
-
 Kloeckera sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
 2 * 31000 SDS-PAGE Kloeckera sp.
58000
-
 gel filtration Kloeckera sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-formylglutathione + H2O [Candida] boidinii
-
 ?
-
 ?
S-formylglutathione + H2O Kloeckera sp. involved in the oxidation of methanol by methylotrophic yeasts ?
-
 ?

Organism

Organism UniProt Comment Textmining
Kloeckera sp.
-
 No.2201, grown in presence of methanol
-
Ogataea angusta
-
-
-
Ogataea trehalophila
-
-
-
Torulopsis pinus
-
-
-
[Candida] boidinii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Kloeckera sp.

Storage Stability

Storage Stability Organism
-20°C Kloeckera sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-formylglutathione + H2O
-
 [Candida] boidinii glutathione + formate
-
 ?
S-formylglutathione + H2O
-
 Ogataea angusta glutathione + formate
-
 ?
S-formylglutathione + H2O
-
 Kloeckera sp. glutathione + formate
-
 ?
S-formylglutathione + H2O
-
 Ogataea trehalophila glutathione + formate
-
 ?
S-formylglutathione + H2O
-
 Torulopsis pinus glutathione + formate
-
 ?
S-formylglutathione + H2O
-
 [Candida] boidinii ?
-
 ?
S-formylglutathione + H2O involved in the oxidation of methanol by methylotrophic yeasts Kloeckera sp. ?
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 [Candida] boidinii
dimer 2 * 31000 SDS-PAGE Kloeckera sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Kloeckera sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
-
 Kloeckera sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4 6.6
-
 Kloeckera sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.2 7.6 5°C, stable for 4 d Kloeckera sp.