BRENDA - Enzyme Database show
show all sequences of 3.1.2.12

Purification, crystallization and preliminary X-ray diffraction analysis of S-formylglutathione hydrolase from Arabidopsis thaliana: effects of pressure and selenomethionine substitution on space-group changes

McAuley, K.E.; Cummins, I.; Papiz, M.; Edwards, R.; Fordham-Skelton, A.P.; Acta Crystallogr. Sect. D 59, 2272-2274 (2003)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression as His-tagged enzyme in Escherichia coli BL21(DE3), production of a selenomethionine labelled enzyme in a methionine auxotrophic strain
Arabidopsis thaliana
Crystallization (Commentary)
Crystallization
Organism
selenomethionine labelled recombinant enzyme, 30 mg/ml, hanging-drop vapor-diffusion method, 293K, precipitant solution: 16% PEG 4000, 0.2 M magnesium acetate, 0.2 M imidazole-malate, pH 6.5, 3% methanol, optimal crystals by microseeding, 1-5 d, production of selenomethionine-enzyme crystals is similar with 10% PEG 4000 and additional presence of 10 mM DTT, X-ray diffraction structure determination and analysis at 1.84 A resolution
Arabidopsis thaliana
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-formylglutathione + H2O
Arabidopsis thaliana
key enzyme catalyzes the final step of the formaldehyde detoxification, glutathione recycling
glutathione + formate
-
Arabidopsis thaliana
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
-
-
-
Purification (Commentary)
Commentary
Organism
recombinant from overexpressing Escherichia coli BL21(DE3) as His-tagged enzyme
Arabidopsis thaliana
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-formylglutathione + H2O
-
649219
Arabidopsis thaliana
glutathione + formate
-
649219
Arabidopsis thaliana
?
S-formylglutathione + H2O
key enzyme catalyzes the final step of the formaldehyde detoxification, glutathione recycling
649219
Arabidopsis thaliana
glutathione + formate
-
649219
Arabidopsis thaliana
?
Cloned(Commentary) (protein specific)
Commentary
Organism
expression as His-tagged enzyme in Escherichia coli BL21(DE3), production of a selenomethionine labelled enzyme in a methionine auxotrophic strain
Arabidopsis thaliana
Crystallization (Commentary) (protein specific)
Crystallization
Organism
selenomethionine labelled recombinant enzyme, 30 mg/ml, hanging-drop vapor-diffusion method, 293K, precipitant solution: 16% PEG 4000, 0.2 M magnesium acetate, 0.2 M imidazole-malate, pH 6.5, 3% methanol, optimal crystals by microseeding, 1-5 d, production of selenomethionine-enzyme crystals is similar with 10% PEG 4000 and additional presence of 10 mM DTT, X-ray diffraction structure determination and analysis at 1.84 A resolution
Arabidopsis thaliana
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
S-formylglutathione + H2O
Arabidopsis thaliana
key enzyme catalyzes the final step of the formaldehyde detoxification, glutathione recycling
glutathione + formate
-
Arabidopsis thaliana
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant from overexpressing Escherichia coli BL21(DE3) as His-tagged enzyme
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
S-formylglutathione + H2O
-
649219
Arabidopsis thaliana
glutathione + formate
-
649219
Arabidopsis thaliana
?
S-formylglutathione + H2O
key enzyme catalyzes the final step of the formaldehyde detoxification, glutathione recycling
649219
Arabidopsis thaliana
glutathione + formate
-
649219
Arabidopsis thaliana
?
Other publictions for EC 3.1.2.12
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
751775
Xu
Regulation of S-formylglutath ...
Homo sapiens, Mus musculus
Oncotarget
8
88259-88275
2017
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Esterase D enhances type I in ...
Sus scrofa
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Huetten
Activity profiling reveals ch ...
Arabidopsis thaliana
Plant Physiol. Biochem.
97
36-43
2015
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731220
Legler
A role for His-160 in peroxide ...
Saccharomyces cerevisiae
Arch. Biochem. Biophys.
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7-20
2012
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1
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1
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9
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2
3
13
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2
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1
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15
1
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Parracino
Towards nanoscale biomedical d ...
Pseudoalteromonas haloplanktis, Pseudoalteromonas haloplanktis TAC 125
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663-672
2011
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707759
Alterio
Crystal structure of an S-form ...
Pseudoalteromonas haloplanktis, Pseudoalteromonas haloplanktis TAC 125
Biopolymers
93
669-677
2010
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1
1
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2
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11
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1
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8
1
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Liu
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Pseudomonas putida
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2010
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Activity-based protein profili ...
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694178
Kaschani
Diversity of serine hydrolase ...
Arabidopsis thaliana
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1082-1093
2009
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6
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1
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6
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Structural characterization an ...
Saccharomyces cerevisiae
Biochemistry
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3
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2
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24
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24
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Structure activity studies wit ...
Arabidopsis thaliana
Phytochemistry
68
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2007
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1
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14
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6
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14
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14
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682315
Cummins
Identification of a carboxyles ...
Arabidopsis thaliana
Plant Biotechnol. J.
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354-359
2007
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Molecular basis of formaldehyd ...
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1
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31
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1
1
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1
1
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15
1
1
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19
1
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1
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1
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9
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2
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17
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2
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1
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1
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15
1
1
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19
1
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666081
Cummins
Unique regulation of the activ ...
Arabidopsis thaliana
J. Mol. Biol.
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2006
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1
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4
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666299
Stover
-
Reciprocal fusions of two gene ...
Phaeodactylum tricornutum, Sterkiella histriomuscorum, Tetrahymena thermophila, Thalassiosira pseudonana
Mol. Biol. Evol.
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1749
2005
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4
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649219
McAuley
Purification, crystallization ...
Arabidopsis thaliana
Acta Crystallogr. Sect. D
59
2272-2274
2003
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1
1
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653164
Yurimoto
Physiological role of S-formyl ...
Candida boidinii
Microbiology
149
1971-1979
2003
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1
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1
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649455
Kordic
Cloning and characterization o ...
Arabidopsis thaliana
Arch. Biochem. Biophys.
399
232-238
2002
1
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4
7
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2
1
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5
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1
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653549
Haslam
-
Cloning and characterisation o ...
Arabidopsis thaliana
Plant Physiol. Biochem.
40
281-288
2002
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2
2
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1
1
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1
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1
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1
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5
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80990
Degrassi
Purification and properties of ...
Saccharomyces cerevisiae
Appl. Environ. Microbiol.
65
3470-3472
1999
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2
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1
1
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2
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6
1
1
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1
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2
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1
1
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1
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6
1
1
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1
-
-
-
-
-
-
-
-
-
80992
Uotila
Expression of formaldehyde deh ...
Rattus norvegicus
Adv. Exp. Med. Biol.
414
365-371
1997
-
-
-
-
-
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1
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2
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15
15
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1
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1
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15
15
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1
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-
-
80991
Harms
S-Formylglutathione hydrolase ...
Paracoccus denitrificans
J. Bacteriol.
178
6296-6299
1996
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-
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1
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6
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80993
Koivusalo
Purification and characterizat ...
Homo sapiens, Lota maculosa, Rattus norvegicus
Adv. Exp. Med. Biol.
372
427-434
1995
-
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3
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2
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6
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3
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3
3
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10
1
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-
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3
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2
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-
-
3
-
3
3
-
10
1
-
-
-
-
-
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-
-
-
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-
-
-
-
80989
Allais
-
Oxidation of methanol by the y ...
Komagataella pastoris, Komagataella pastoris IFP 206
Agric. Biol. Chem.
47
1509-1516
1983
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-
-
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4
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2
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-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
80988
Kato
-
Oxidation of formaldehyde by r ...
Geotrichum klebahnii, Kloeckera sp., Schwanniomyces vanrijiae
Agric. Biol. Chem.
46
655-661
1982
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
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-
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3
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-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
80987
Uotila
S-Formylglutathione hydrolase ...
Escherichia coli, Homo sapiens
Methods Enzymol.
77
320-325
1981
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-
-
-
-
1
6
2
-
-
2
-
-
2
-
-
1
-
-
1
1
-
3
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
1
-
6
-
2
-
-
2
-
-
-
-
1
-
1
1
-
3
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
80985
Kato
Purificaton and characterizati ...
Candida boidinii, Kloeckera sp., Ogataea angusta, Ogataea trehalophila, Torulopsis pinus
Biochim. Biophys. Acta
611
323-332
1980
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-
-
-
-
-
10
1
-
-
2
2
-
7
-
-
1
-
-
-
-
1
7
2
1
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
10
-
1
-
-
2
2
-
-
-
1
-
-
-
1
7
2
1
-
1
-
1
-
1
-
-
-
-
-
-
-
80986
Neben
Studies on an enzyme, S-formyl ...
Candida boidinii
Biochim. Biophys. Acta
614
81-91
1980
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-
-
-
-
-
1
1
-
-
3
1
-
2
-
-
1
-
-
-
1
1
3
1
-
-
1
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-
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-
-
-
-
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1
-
1
-
-
3
1
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-
1
-
-
1
1
3
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
80983
Uotila
Purification of formaldehyde a ...
Pisum sativum
Arch. Biochem. Biophys.
196
33-45
1979
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-
-
-
-
-
-
-
-
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-
1
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1
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1
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1
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1
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1
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
80984
Uotila
Glutathione thiol esterases of ...
Homo sapiens
Biochim. Biophys. Acta
580
277-288
1979
-
-
-
-
-
-
-
-
-
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-
-
2
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1
2
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1
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1
2
-
1
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
80982
Uotila
Purification and properties of ...
Homo sapiens
J. Biol. Chem.
249
7664-7672
1974
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-
-
-
-
1
14
2
1
-
3
-
-
2
-
-
1
-
-
2
1
1
2
1
1
-
-
-
1
-
1
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-
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-
-
-
-
-
-
1
-
14
-
2
1
-
3
-
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-
-
1
-
2
1
1
2
1
1
-
-
-
1
-
1
-
-
-
-
-
-
-
80981
Uotila
Preparation and assay of gluta ...
Homo sapiens
Biochemistry
12
3938-3943
1973
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-
-
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1
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1
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1
1
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1
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1
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2
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1
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1
1
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1
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1
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2
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