Literature summary for 3.1.16.1 extracted from

Choudhury, S.A.; Asefa, B.; Webb, A.; Ramotar, D.; Chow, T.Y.
Functional and genetic analysis of the Saccharomyces cerevisiae RNC1/TRM2: evidences for its involvement in DNA double-strand break repair (2007), Mol. Cell. Biochem., 300, 215-226.

Search for more literature for 3.1.16.1

Application

Application	Comment	Organism
additional information	important role for TRM2 in DNA repair with a potential involvement of its nuclease function in homologous recombination based repair of DNA double-strand breaks, plays no role in the nucleotide excision repair and/or base excision repair pathways	Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment)	Organism
Escherichia coli BL21-Gold DE3 transformed with the plasmid pCBP-TRM2 and pCBP-DTRM2(ctdelta1689-1920nt), also cloned into a GFP vector bearing a GAL1::GFP transcriptional fusion	Saccharomyces cerevisiae

Protein Variants

Protein Variants	Comment	Organism
additional information	Trm2p(ctdelta76aa), lacks 76 amino acids at the C-terminus, retains nuclease activities but not the methyl transferase activity. Trm2(delta232-1920nt) mutant, contains only the first 231 nucleotides of the TRM2 gene, displays low sensitivity to methyl methane sulfonate and suppresses the methyl methane sulfonate sensitivity of rad52 mutants in trm2(delta232-1920nt)rad52 double mutants. Trm2 exo1 double mutants are synergistically more sensitive to methyl methane sulfonate and ionizing radiation than either of the single mutant. The ku80trm2(deltaD232-1920nt) double mutant displays much higher methyl methane sulfonate sensitivity than either of the single mutant alone. The trm2 (delta232-1920nt) single mutant conferrs only a very slight sensitivity to gamma irradiation, while the trm2(delta232-1920nt)exo1 double mutant exhibits 1.7-2.2fold higher sensitivity relative to trm2(delta232-1920nt) mutant	Saccharomyces cerevisiae

Inhibitors

Inhibitors	Comment	Organism	Structure
Pentamidine	endo-exonuclease inhibitor, inhibits DNase activity of CBP-Trm2p	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
by calmodulin affinity chromatography	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
DNA + H2O	Trm2p displays 5' to 3' exonuclease activity on double-strand DNA, and endonuclease activity on single-strand DNA	Saccharomyces cerevisiae	deoxyribonucleoside 3'-phosphates	-	?

Synonyms

Synonyms	Comment	Organism
Trm2p	-	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)