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Literature summary for 3.1.16.1 extracted from

  • Dolapchiev, L.B.; Bakalova, A.
    Simple purification and some properties of beef spleen exonuclease (1982), Prep. Biochem., 12, 121-136.
    View publication on PubMed
Search for more literature for 3.1.16.1

Application

Application Comment Organism
analysis kinetic studies on macromolecular substrates degradation Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
Urea after 2 h incubation almost 80% of activity with 1.0 M, 40% with 2.0 M, no activity with 5.0 M Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
98000
-
 molecular sieving, SDS gel electrophoresis, sucrose density gradient centrifugation Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
no endonuclease and phophomonoesterase activities Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
spleen
-
 Bos taurus
-

Storage Stability

Storage Stability Organism
4°C, lyophilized crude enzyme, more than 1 year, no loss of activity Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
RNA core + H2O
-
 Bos taurus nucleoside 3'-phosphates
-
 ?
thymidine 3'-(4-nitrophenyl)phosphate + H2O
-
 Bos taurus thymidine 3'-phosphate + 4-nitrophenol
-
 ?

Synonyms

Synonyms Comment Organism
phosphodiesterase II
-
 Bos taurus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35 60 incubation at pH 5.7, 10 min: 90% of initial activity at 35°C, 10% at 60°C Bos taurus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 7 incubation of pure enzyme for 2 h at 4°C, beyond the limits sharp decrease of stability Bos taurus