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Literature summary for 3.1.13.4 extracted from

  • Niedzwiecka, A.; Lekka, M.; Nilsson, P.; Virtanen, A.
    Global architecture of human poly(A)-specific ribonuclease by atomic force microscopy in liquid and dynamic light scattering (2011), Biophys. Chem., 158, 141-149.
    View publication on PubMed
Search for more literature for 3.1.13.4

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged full-length enzyme, comprising residues 1-639, and His-tagged enzyme fragment, PARN(443-560), containing the RNA recognition motif with a 50 amino acids long C-terminal tail, comprising residues 443-560, are expressed in Escherichia coli strain BL21(DE3) Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
K+ required Homo sapiens
Mg2+ required Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
17000
-
 2 * 17000, SDS-PAGE, dimerization of enzyme fragment PARN(443-560) Homo sapiens
37000
-
 dimerization of enzyme fragment PARN(443-560), gel filtration Homo sapiens
76200
-
 x * 76200, His-tagged full-length enzyme, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens Poly(A)-specific ribonuclease is a eukaryotic enzyme that efficiently degrades mRNA poly(A) tails ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q96LI5
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged full-length enzyme and His-tagged enzyme fragment from Escherichia coli strain BL21(DE3) Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information Poly(A)-specific ribonuclease is a eukaryotic enzyme that efficiently degrades mRNA poly(A) tails Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
? x * 76200, His-tagged full-length enzyme, SDS-PAGE Homo sapiens
dimer 2 * 17000, SDS-PAGE, dimerization of enzyme fragment PARN(443-560) Homo sapiens
More the human enzyme is composed of at least three functional domains, i.e. the catalytic nuclease domain and two RNA binding domains, the R3H and the RNA recognition motif, respectively, detailed analysis of the global architecture of the human enzyme and dimensions of the full-length protein at subnanometer resolution, by atomic force microscopy imaging, overview Homo sapiens

Synonyms

Synonyms Comment Organism
PARN
-
 Homo sapiens
poly(A)-specific ribonuclease
-
 Homo sapiens