Cloned (Comment) | Organism |
---|---|
expressed as N-terminal His6-tagged ORN enzyme under control of the bacteriophage T7 promoter in Escherichia coli BL21(kDE3)/pLysS using derivatives of the expression vector pETMCSI | Escherichia coli |
expressed as N-terminal His6-tagged ORN enzyme under control of the bacteriophage T7 promoter in Escherichia coli BL21(kDE3)/pLysS using derivatives of the expression vector pETMCSI | Homo sapiens |
expressed as N-terminal His6-tagged ORN enzyme under control of the bacteriophage T7 promoter in Escherichia coli BL21(kDE3)/pLysS using derivatives of the expression vector pETMCSI | Mycolicibacterium smegmatis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ni2+ | effective competitive inhibitor | Escherichia coli | |
Ni2+ | effective competitive inhibitor | Homo sapiens | |
Ni2+ | effective competitive inhibitor | Mycolicibacterium smegmatis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.27 | - |
5'-p-nitrophenyl-TMP | in the presence of 3 mM Mn2+ | Homo sapiens | |
0.28 | - |
5'-p-nitrophenyl-TMP | in the presence of 3 mM Mn2+ | Mycolicibacterium smegmatis | |
0.46 | - |
5'-p-nitrophenyl-TMP | in the presence of 1 mM Mn2+ | Mycolicibacterium smegmatis | |
0.54 | - |
5'-p-nitrophenyl-TMP | in the presence of 3 mM Mn2+ | Escherichia coli | |
0.85 | - |
5'-p-nitrophenyl-TMP | in the presence of 1 mM Mn2+ | Homo sapiens | |
1.9 | - |
5'-p-nitrophenyl-TMP | in the presence of 1 mM Mn2+ | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Escherichia coli | |
Mg2+ | - |
Homo sapiens | |
Mg2+ | - |
Mycolicibacterium smegmatis | |
Mn2+ | preferred over Mg2+ | Escherichia coli | |
Mn2+ | preferred over Mg2+ | Homo sapiens | |
Mn2+ | preferred over Mg2+ | Mycolicibacterium smegmatis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21638 | - |
2 * 21638, electrospray ionisation mass spectrometry | Escherichia coli |
24576 | - |
2 * 24576, electrospray ionisation mass spectrometry | Homo sapiens |
24637 | - |
2 * 24637, electrospray ionisation mass spectrometry | Mycolicibacterium smegmatis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Homo sapiens | - |
- |
- |
Mycolicibacterium smegmatis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
on Ni-NTA column and by gel filtration, more than 95% pure | Escherichia coli |
on Ni-NTA column and by gel filtration, more than 95% pure | Homo sapiens |
on Ni-NTA column and by gel filtration, more than 95% pure | Mycolicibacterium smegmatis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.77 | - |
purified enzyme | Escherichia coli |
7.1 | - |
purified enzyme | Homo sapiens |
14.9 | - |
purified enzyme | Mycolicibacterium smegmatis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-p-nitrophenyl-TMP + H2O | - |
Escherichia coli | p-nitrophenol + TMP | - |
? | |
5'-p-nitrophenyl-TMP + H2O | - |
Homo sapiens | p-nitrophenol + TMP | - |
? | |
5'-p-nitrophenyl-TMP + H2O | - |
Mycolicibacterium smegmatis | p-nitrophenol + TMP | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 21638, electrospray ionisation mass spectrometry | Escherichia coli |
homodimer | 2 * 24576, electrospray ionisation mass spectrometry | Homo sapiens |
homodimer | 2 * 24637, electrospray ionisation mass spectrometry | Mycolicibacterium smegmatis |
Synonyms | Comment | Organism |
---|---|---|
ORN | - |
Escherichia coli |
ORN | - |
Homo sapiens |
ORN | - |
Mycolicibacterium smegmatis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Escherichia coli |
25 | - |
- |
Homo sapiens |
25 | - |
- |
Mycolicibacterium smegmatis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | 0.55 | 5'-p-nitrophenyl-TMP | in the presence of 3 mM Mn2+ | Homo sapiens | |
0.04 | 1.97 | 5'-p-nitrophenyl-TMP | in the presence of 1 mM Mn2+ | Homo sapiens | |
0.04 | 1.97 | 5'-p-nitrophenyl-TMP | in the presence of 3 mM Mn2+ | Mycolicibacterium smegmatis | |
0.057 | 0.65 | 5'-p-nitrophenyl-TMP | in the presence of 1 mM Mn2+ | Mycolicibacterium smegmatis | |
2.15 | - |
5'-p-nitrophenyl-TMP | in the presence of 1 mM Mn2+ | Escherichia coli | |
2.3 | - |
5'-p-nitrophenyl-TMP | in the presence of 3 mM Mn2+ | Escherichia coli | |
7.417 | - |
5'-p-nitrophenyl-TMP | in the presence of 3 mM Mn2+ | Homo sapiens | |
8.767 | - |
5'-p-nitrophenyl-TMP | in the presence of 1 mM Mn2+ | Homo sapiens | |
9.317 | - |
5'-p-nitrophenyl-TMP | in the presence of 3 mM Mn2+ | Mycolicibacterium smegmatis | |
10.88 | - |
5'-p-nitrophenyl-TMP | in the presence of 1 mM Mn2+ | Mycolicibacterium smegmatis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Escherichia coli |
8 | - |
- |
Homo sapiens |
8 | - |
- |
Mycolicibacterium smegmatis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
Ni2+ | - |
Homo sapiens | |
0.039 | - |
Ni2+ | - |
Escherichia coli | |
0.24 | - |
Ni2+ | - |
Mycolicibacterium smegmatis |