Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | supernatant fraction | Escherichia coli | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | no stimulation | Escherichia coli | |
K+ | no effect | Escherichia coli | |
Mg2+ | activation | Escherichia coli | |
Mn2+ | strong stimulation | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
oligonucleotides + H2O | Escherichia coli | - |
nucleoside 5'-phosphates | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10.75 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-25°C, 50% glycerol | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | no activity | Escherichia coli | nucleoside 5'-phosphates | - |
? | |
NAD+ + H2O | - |
Escherichia coli | NMN + AMP | - |
? | |
oligonucleotides + H2O | - |
Escherichia coli | nucleoside 5'-phosphates | - |
? | |
oligonucleotides + H2O | exonuclease: 3`to 5'-direction | Escherichia coli | nucleoside 5'-phosphates | - |
? | |
oligonucleotides + H2O | reaction rate inversely proportional to chain length of substrate | Escherichia coli | nucleoside 5'-phosphates | - |
? | |
oligonucleotides + H2O | specific for short ribooligonucleotides | Escherichia coli | nucleoside 5'-phosphates | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
half-life: 10 min | Escherichia coli |
70 | - |
half-life: 10 min | Escherichia coli |
100 | - |
half-life: 2.5 min | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | - |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9.5 | pH 7.0: 50% of maximum activity, pH 9.5: 70% of maximum activity | Escherichia coli |