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Literature summary for 3.1.11.2 extracted from
- A deoxyribonucleic acid phosphatase-exonuclease from Escherichia coli (1964), J. Biol. Chem., 239, 251-258.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| p-chloromercuribenzoate | 50% inhibition of DNA phosphatase activity, 90% loss of exonuclease activity | Escherichia coli |  |
| ZnCl2 | 0.03 mM, 90% inhibition of DNA phosphatase activity and 88% loss of exonuclease activity | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 3 mM CaCl2 | Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| double-stranded DNA + H2O | 3'-phosphoryl-terminated DNA | Escherichia coli | additional information | 5'-mononucleotides + phosphate + large molecular weight single stranded oligonucleotides | ? | |
| double-stranded DNA + H2O | heat-denatured E. coli DNA | Escherichia coli | additional information | 5'-mononucleotides + phosphate + large molecular weight single stranded oligonucleotides | ? | |
| double-stranded DNA + H2O | 3'-hydroxyl-terminated DNA | Escherichia coli | additional information | 5'-mononucleotides + phosphate + large molecular weight single stranded oligonucleotides | ? | |
| double-stranded DNA + H2O | dAT copolymer | Escherichia coli | additional information | 5'-mononucleotides + phosphate + large molecular weight single stranded oligonucleotides | ? | |
| double-stranded DNA + H2O | double stranded DNA | Escherichia coli | additional information | 5'-mononucleotides + phosphate + large molecular weight single stranded oligonucleotides | ? | |
| double-stranded DNA + H2O | native E. coli DNA | Escherichia coli | additional information | 5'-mononucleotides + phosphate + large molecular weight single stranded oligonucleotides | ? | |
| double-stranded DNA + H2O | native Bacillus subtilis DNA | Escherichia coli | additional information | 5'-mononucleotides + phosphate + large molecular weight single stranded oligonucleotides | ? | |
| double-stranded DNA + H2O | up to 40% degradation, residual single stranded chains are resistant | Escherichia coli | additional information | 5'-mononucleotides + phosphate + large molecular weight single stranded oligonucleotides | ? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
30 min, in absence of Mg2+ or substrate, 93% loss of activity | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.7 | 8.4 | - |
Escherichia coli |
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Release 2023.1 (January 2023)
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