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Literature summary for 3.1.1.85 extracted from
- Expression and activity of the BioH esterase of biotin synthesis is independent of genome context (2017), Sci. Rep., 7, 2141 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Pseudomonas aeruginosa PAO1 |
| expression in Escherichia coli | Escherichia coli K-12 |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pimeloyl-[acyl-carrier protein] methyl ester + H2O | Pseudomonas aeruginosa PAO1 | involved in biotin biosynthesis | pimeloyl-[acyl-carrier protein] + methanol | - |
? |  |
| pimeloyl-[acyl-carrier protein] methyl ester + H2O | Escherichia coli K-12 | involved in biotin biosynthesis | pimeloyl-[acyl-carrier protein] + methanol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli K-12 | P13001 | - |
- |
| Pseudomonas aeruginosa PAO1 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| N-terminal hexahistidine-tagged enzyme | Pseudomonas aeruginosa PAO1 |
| N-terminal hexahistidine-tagged enzyme | Escherichia coli K-12 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| adipyl-[acyl-carrier protein] methyl ester + H2O | - |
Pseudomonas aeruginosa PAO1 | adipyl-[acyl-carrier protein] + methanol | - |
? | |
| adipyl-[acyl-carrier protein] methyl ester + H2O | - |
Escherichia coli K-12 | adipyl-[acyl-carrier protein] + methanol | - |
? | |
| azelayl-[acyl-carrier protein] methyl ester + H2O | - |
Pseudomonas aeruginosa PAO1 | azelayl-[acyl-carrier protein] + methanol | - |
? | |
| azelayl-[acyl-carrier protein] methyl ester + H2O | - |
Escherichia coli K-12 | azelayl-[acyl-carrier protein] + methanol | - |
? | |
| additional information | no activity with succinyl-[acyl-carrier protein] methyl ester | Pseudomonas aeruginosa PAO1 | ? | - |
? | |
| additional information | no activity with succinyl-[acyl-carrier protein] methyl ester | Escherichia coli K-12 | ? | - |
? | |
| pimeloyl-[acyl-carrier protein] methyl ester + H2O | - |
Pseudomonas aeruginosa PAO1 | pimeloyl-[acyl-carrier protein] + methanol | - |
? | |
| pimeloyl-[acyl-carrier protein] methyl ester + H2O | - |
Escherichia coli K-12 | pimeloyl-[acyl-carrier protein] + methanol | - |
? | |
| pimeloyl-[acyl-carrier protein] methyl ester + H2O | involved in biotin biosynthesis | Pseudomonas aeruginosa PAO1 | pimeloyl-[acyl-carrier protein] + methanol | - |
? | |
| pimeloyl-[acyl-carrier protein] methyl ester + H2O | involved in biotin biosynthesis | Escherichia coli K-12 | pimeloyl-[acyl-carrier protein] + methanol | - |
? | |
| suberyl-[acyl-carrier protein] methyl ester + H2O | - |
Pseudomonas aeruginosa PAO1 | suberyl-[acyl-carrier protein] + methanol | - |
? | |
| suberyl-[acyl-carrier protein] methyl ester + H2O | - |
Escherichia coli K-12 | suberyl-[acyl-carrier protein] + methanol | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BioH | - |
Pseudomonas aeruginosa PAO1 |
| BioH | - |
Escherichia coli K-12 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Pseudomonas aeruginosa PAO1 |
| 37 | - |
assay at | Escherichia coli K-12 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Pseudomonas aeruginosa PAO1 |
| 7 | - |
assay at | Escherichia coli K-12 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | involved in biotin biosynthesis, in Escherichia coli the bioh gene is not located within a biotin synthetic operon and its transcription is not coregulated with the other biotin synthesis genes. Operon-encoded bioH in Pseudomonas aeruginosa is transcribed at higher levels than the freestanding bioH in Escherichia coli | Escherichia coli K-12 |
| metabolism | involved in biotin biosynthesis, in Pseudomonas aeruginosa the bioH gene is within a biotin synthesis operon and its transcription is coregulated with the other biotin operon genes. Operon-encoded bioH in Pseudomonas aeruginosa is transcribed at higher levels than the freestanding bioH in Escherichia coli | Pseudomonas aeruginosa PAO1 |
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