Cloned (Comment) | Organism |
---|---|
enzyme CocH1, the A199S/F227A/S287G/A328W mutant of human BChE (EC 3.1.1.8) containing C-terminal human serum albumin (HSA) is generated and cloned in to pCMV-MCS and expressed in CHO-S cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | potential inhibitory activity of heroin or 6-monoacetylmorphine against CocH1-catalyzed hydrolysis of another substrate like (-)-cocaine | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0031 | - |
(-)-cocaine | recombinant enzyme, pH 7.4, 37°C | Homo sapiens | |
0.245 | - |
heroin | recombinant enzyme, pH 7.4, 37°C | Homo sapiens | |
0.292 | - |
6-monoacetylmorphine | recombinant enzyme, pH 7.4, 37°C | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-monoacetylmorphine + H2O | Homo sapiens | - |
morphine + acetate | - |
? | |
heroin + H2O | Homo sapiens | - |
6-monoacetylmorphine + acetate | - |
? | |
additional information | Homo sapiens | heroin hydrolysis to 6-MAM and morphine is accelerated by cholinesterases, including acetylcholinesterase (AChE, EC 3.1.1.7) and/or butyrylcholinesterase (BChE, EC 3.1.1.8) | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O00748 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(-)-cocaine + H2O | - |
Homo sapiens | ecgonine methyl ester + benzoate | - |
? | |
6-monoacetylmorphine + H2O | - |
Homo sapiens | morphine + acetate | - |
? | |
heroin + H2O | - |
Homo sapiens | 6-monoacetylmorphine + acetate | - |
? | |
additional information | heroin hydrolysis to 6-MAM and morphine is accelerated by cholinesterases, including acetylcholinesterase (AChE, EC 3.1.1.7) and/or butyrylcholinesterase (BChE, EC 3.1.1.8) | Homo sapiens | ? | - |
? | |
additional information | the enzyme activity for converting 6-monoacetylmorphine to morphine is much lower than that for converting heroin to 6-monoacetylmorphine. Substrate specificities compared to acetylcholinesterase (EC 3.1.1.7) and butyrylcholinesterase (EC 3.1.1.8) | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
6-MAM hydrolyse | - |
Homo sapiens |
cocaine hydrolase | - |
Homo sapiens |
CocH1 | - |
Homo sapiens |
heroin hydrolyse | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0037 | - |
6-monoacetylmorphine | recombinant enzyme, pH 7.4, 37°C | Homo sapiens | |
35.8 | - |
heroin | recombinant enzyme, pH 7.4, 37°C | Homo sapiens | |
51 | - |
(-)-cocaine | recombinant enzyme, pH 7.4, 37°C | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics, kinetic modelling, overview | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
additional information | enzyme structure homology modelling, molecular dynamics simulations on the structures of enzyme-substrate complexes using the crystal structures of AChE (PDB ID 1B41), and BChE (PDB IDs 2XQF and 1P0P), molecular docking, overview. The positively charged amino-group of the substrates (heroin and 6-monoacetylmorphine) is placed in the choline-binding site near Trp82 in BChE and CocH1 or Trp86 in AChE. The binding models of heroin and 6-monoacetylmorphine in the corresponding enzyme-substrate complexes are optimized by performing the energy minimization | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0005 | - |
6-monoacetylmorphine | recombinant enzyme, pH 7.4, 37°C | Homo sapiens | |
0.013 | - |
heroin | recombinant enzyme, pH 7.4, 37°C | Homo sapiens | |
16452 | - |
(-)-cocaine | recombinant enzyme, pH 7.4, 37°C | Homo sapiens |