BRENDA - Enzyme Database show
show all sequences of 3.1.1.84

Identification of a cocaine esterase in a strain of Pseudomonas maltophilia

Britt, A.J.; Bruce, N.C.; Lowe, C.R.; J. Bacteriol. 174, 2087-2094 (1992)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
no inhibition by 1 mM eserine or 1 mM p-hydroxymercuribenzoate. Cocaine esterase shows product inhibition with neither benzoate nor ecgonine methyl ester
Stenotrophomonas maltophilia
phenylmethylsulfonyl fluoride
1 mM, complete inhibition
Stenotrophomonas maltophilia
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.36
-
cocaine
pH 7.0, 30°C
Stenotrophomonas maltophilia
1.75
-
ethyl 2-hydroxybenzoate
pH 7.0, 30°C
Stenotrophomonas maltophilia
1.89
-
ethyl benzoate
pH 7.0, 30°C
Stenotrophomonas maltophilia
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
110000
-
in the presence of the solubilizing agent cholate, gel filtration
Stenotrophomonas maltophilia
129000
-
1 * 129000, SDS-PAGE
Stenotrophomonas maltophilia
410000
-
in the absence of cholate, cocaine esterase has a native MW of 410000 and probably existed as a tetramer, gel filtration
Stenotrophomonas maltophilia
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
cocaine + H2O
Stenotrophomonas maltophilia
strain MB11L of Pseudomonas maltophilia is capable of using cocaine as its sole carbon and energy source
ecgonine methyl ester + benzoate
-
-
?
cocaine + H2O
Stenotrophomonas maltophilia MB11L
strain MB11L of Pseudomonas maltophilia is capable of using cocaine as its sole carbon and energy source
ecgonine methyl ester + benzoate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Stenotrophomonas maltophilia
-
-
-
Stenotrophomonas maltophilia MB11L
-
-
-
Purification (Commentary)
Commentary
Organism
partial
Stenotrophomonas maltophilia
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5
-
-
Stenotrophomonas maltophilia
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
cocaine + H2O
-
698540
Stenotrophomonas maltophilia
ecgonine methyl ester + benzoate
-
-
-
?
cocaine + H2O
strain MB11L of Pseudomonas maltophilia is capable of using cocaine as its sole carbon and energy source
698540
Stenotrophomonas maltophilia
ecgonine methyl ester + benzoate
-
-
-
?
cocaine + H2O
-
698540
Stenotrophomonas maltophilia MB11L
ecgonine methyl ester + benzoate
-
-
-
?
cocaine + H2O
strain MB11L of Pseudomonas maltophilia is capable of using cocaine as its sole carbon and energy source
698540
Stenotrophomonas maltophilia MB11L
ecgonine methyl ester + benzoate
-
-
-
?
ethyl 2-hydroxybenzoate + H2O
-
698540
Stenotrophomonas maltophilia
ethanol + 2-hydroxybenzoate
-
-
-
?
ethyl 2-hydroxybenzoate + H2O
-
698540
Stenotrophomonas maltophilia MB11L
ethanol + 2-hydroxybenzoate
-
-
-
?
ethyl benzoate + H2O
-
698540
Stenotrophomonas maltophilia
ethanol + benzoate
-
-
-
?
ethyl benzoate + H2O
-
698540
Stenotrophomonas maltophilia MB11L
ethanol + benzoate
-
-
-
?
additional information
no activity with: atropine, ethyl 3-hydroxybenzoate or ethyl 4-hydroxybenzoate
698540
Stenotrophomonas maltophilia
?
-
-
-
-
additional information
no activity with: atropine, ethyl 3-hydroxybenzoate or ethyl 4-hydroxybenzoate
698540
Stenotrophomonas maltophilia MB11L
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
monomer
1 * 129000, SDS-PAGE
Stenotrophomonas maltophilia
tetramer
in the absence of cholate, cocaine esterase has a native MW of 410000 and probably existed as a tetramer
Stenotrophomonas maltophilia
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Stenotrophomonas maltophilia
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Stenotrophomonas maltophilia
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
no inhibition by 1 mM eserine or 1 mM p-hydroxymercuribenzoate. Cocaine esterase shows product inhibition with neither benzoate nor ecgonine methyl ester
Stenotrophomonas maltophilia
phenylmethylsulfonyl fluoride
1 mM, complete inhibition
Stenotrophomonas maltophilia
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.36
-
cocaine
pH 7.0, 30°C
Stenotrophomonas maltophilia
1.75
-
ethyl 2-hydroxybenzoate
pH 7.0, 30°C
Stenotrophomonas maltophilia
1.89
-
ethyl benzoate
pH 7.0, 30°C
Stenotrophomonas maltophilia
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
110000
-
in the presence of the solubilizing agent cholate, gel filtration
Stenotrophomonas maltophilia
129000
-
1 * 129000, SDS-PAGE
Stenotrophomonas maltophilia
410000
-
in the absence of cholate, cocaine esterase has a native MW of 410000 and probably existed as a tetramer, gel filtration
Stenotrophomonas maltophilia
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
cocaine + H2O
Stenotrophomonas maltophilia
strain MB11L of Pseudomonas maltophilia is capable of using cocaine as its sole carbon and energy source
ecgonine methyl ester + benzoate
-
-
?
cocaine + H2O
Stenotrophomonas maltophilia MB11L
strain MB11L of Pseudomonas maltophilia is capable of using cocaine as its sole carbon and energy source
ecgonine methyl ester + benzoate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Stenotrophomonas maltophilia
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.5
-
-
Stenotrophomonas maltophilia
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
cocaine + H2O
-
698540
Stenotrophomonas maltophilia
ecgonine methyl ester + benzoate
-
-
-
?
cocaine + H2O
strain MB11L of Pseudomonas maltophilia is capable of using cocaine as its sole carbon and energy source
698540
Stenotrophomonas maltophilia
ecgonine methyl ester + benzoate
-
-
-
?
cocaine + H2O
-
698540
Stenotrophomonas maltophilia MB11L
ecgonine methyl ester + benzoate
-
-
-
?
cocaine + H2O
strain MB11L of Pseudomonas maltophilia is capable of using cocaine as its sole carbon and energy source
698540
Stenotrophomonas maltophilia MB11L
ecgonine methyl ester + benzoate
-
-
-
?
ethyl 2-hydroxybenzoate + H2O
-
698540
Stenotrophomonas maltophilia
ethanol + 2-hydroxybenzoate
-
-
-
?
ethyl 2-hydroxybenzoate + H2O
-
698540
Stenotrophomonas maltophilia MB11L
ethanol + 2-hydroxybenzoate
-
-
-
?
ethyl benzoate + H2O
-
698540
Stenotrophomonas maltophilia
ethanol + benzoate
-
-
-
?
ethyl benzoate + H2O
-
698540
Stenotrophomonas maltophilia MB11L
ethanol + benzoate
-
-
-
?
additional information
no activity with: atropine, ethyl 3-hydroxybenzoate or ethyl 4-hydroxybenzoate
698540
Stenotrophomonas maltophilia
?
-
-
-
-
additional information
no activity with: atropine, ethyl 3-hydroxybenzoate or ethyl 4-hydroxybenzoate
698540
Stenotrophomonas maltophilia MB11L
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 129000, SDS-PAGE
Stenotrophomonas maltophilia
tetramer
in the absence of cholate, cocaine esterase has a native MW of 410000 and probably existed as a tetramer
Stenotrophomonas maltophilia
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Stenotrophomonas maltophilia
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Stenotrophomonas maltophilia
Other publictions for EC 3.1.1.84
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
750291
Kim
Kinetic characterization of c ...
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293
107-114
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3
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5
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1
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3
1
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1
1
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3
3
728856
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Rational design, preparation, ...
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9
1764-1772
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1
1
3
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3
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2
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3
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1
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1
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3
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2
1
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3
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3
3
730250
Brim
The fate of bacterial cocaine ...
Rhodococcus sp.
J. Pharmacol. Exp. Ther.
340
83-95
2012
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1
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1
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730421
Lee
Cell permeable cocaine esteras ...
Rhodococcus sp., Rhodococcus sp. MB1
Mol. Pharm.
9
1361-1373
2012
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1
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5
-
-
6
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1
2
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6
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1
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1
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1
6
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6
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1
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1
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2
1
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1
6
-
-
-
-
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6
6
716278
Xue
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Homo sapiens
Mol. Pharmacol.
79
290-297
2011
-
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1
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3
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4
-
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1
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1
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4
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1
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1
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1
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4
-
-
-
-
-
-
-
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4
4
716281
Narasimhan
Subunit stabilization and pegy ...
Rhodococcus sp.
Mol. Pharmacol.
80
1056-1065
2011
-
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1
1
3
-
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4
-
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1
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3
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1
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4
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1
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3
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4
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1
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1
4
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4
4
714226
Zheng
Design of high-activity mutant ...
Homo sapiens
Biochemistry
49
9113-9119
2010
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1
1
10
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1
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1
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10
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9
9
714702
Yang
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Homo sapiens
Chem. Biol. Interact.
187
148-152
2010
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1
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2
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2
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2
2
716276
Brim
A thermally stable form of bac ...
Rhodococcus sp., Rhodococcus sp. MB1
Mol. Pharmacol.
77
593-600
2010
-
1
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1
1
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4
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7
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1
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716824
Narasimhan
Structural analysis of thermos ...
Rhodococcus sp., Rhodococcus sp. MB1
Protein Eng.
23
537-547
2010
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1
1
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5
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4
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6
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5
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5
5
692028
Ko
Effects of cocaine esterase fo ...
Rhodococcus sp.
Drug Alcohol Depend.
101
202-209
2009
-
1
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694267
Gao
Thermostable variants of cocai ...
Rhodococcus sp.
Mol. Pharmacol.
75
318-323
2009
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1
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4
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3
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1
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1
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699760
Collins
Zhan. C.G.; Woods. J.H.: Cocai ...
Rhodococcus sp.
J. Pharmacol. Exp. Ther.
331
445-455
2009
-
1
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1
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4
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698483
Liu
Fundamental reaction mechanism ...
Rhodococcus sp.
J. Am. Chem. Soc.
131
11964-11975
2003
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650108
Turner
Biochemical characterization a ...
Rhodococcus sp.
Biochemistry
41
12297-12307
2002
-
1
1
1
11
-
-
10
-
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1
1
2
-
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-
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3
-
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9
1
1
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-
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1
1
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1
11
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10
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1
1
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3
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9
1
1
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653322
Larsen
Crystal structure of a bacteri ...
Rhodococcus sp.
Nat. Struct. Biol.
9
17-21
2002
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1
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1
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695729
Bresler
Gene cloning and nucleotide se ...
Rhodococcus sp., Rhodococcus sp. MB1
Appl. Environ. Microbiol.
66
904-908
2000
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1
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1
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2
2
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11
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1
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1
1
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6
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