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Literature summary for 3.1.1.84 extracted from

  • Pindel, E.V.; Kedishvili, N.Y.; Abraham, T.L.; Brzezinski, M.R.; Zhang, J.; Dean, R.A.; Bosron, W.F.
    Purification and cloning of a broad substrate specificity human liver carboxylesterase that catalyzes the hydrolysis of cocaine and heroin (1997), J. Biol. Chem., 272, 14769-14775.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
eserine hCE-2 shows greater inhibition by eserine thann hCE-1 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
6-monoacetylmorphine pH 7.4, 37°C Homo sapiens
0.15
-
4-yethylumbelliferyl acetate pH 7.4, 37°C Homo sapiens
0.39
-
cocaine pH 7.4, 37°C Homo sapiens
6.8
-
heroin pH 7.4, 37°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
1 * 60000, SDS-PAGE Homo sapiens
80000
-
gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cocaine + H2O Homo sapiens hCE-2 exhibits different drug ester substrate specificity from the human liver carboxylesterase hCE-1, which hydrolyzes the methyl ester of cocaine. hCE-2 may play an important role in the degradation of cocaine and heroin in human tissues ecgonine methyl ester + benzoate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens O00748
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein high mannose type Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl acetate + H2O hCE-2 has higher catalytic efficiency for hydrolysis than hCE-1 Homo sapiens 4-methylumbelliferone + acetate
-
?
6-monoacetylmorphine + H2O hCE-2 has higher catalytic efficiency for hydrolysis than hCE-1 Homo sapiens morphine + acetate
-
?
cocaine + H2O hCE-2 exhibits different drug ester substrate specificity from the human liver carboxylesterase hCE-1, which hydrolyzes the methyl ester of cocaine. hCE-2 may play an important role in the degradation of cocaine and heroin in human tissues Homo sapiens ecgonine methyl ester + benzoate
-
?
cocaine + H2O hCE-2 exhibits different drug ester substrate specificity from the human liver carboxylesterase hCE-1, which hydrolyzes the methyl ester of cocaine Homo sapiens ecgonine methyl ester + benzoate
-
?
heroin + H2O hCE-2 has higher catalytic efficiency for hydrolysis than hCE-1 Homo sapiens 6-monoacetylmorphine + acetate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 60000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
hCE-2
-
Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0001
-
eserine
-
Homo sapiens

pI Value

Organism Comment pI Value Maximum pI Value
Homo sapiens isoelectric focusing
-
4.9