BRENDA - Enzyme Database
show all sequences of 3.1.1.84

Purification and cloning of a broad substrate specificity human liver carboxylesterase that catalyzes the hydrolysis of cocaine and heroin

Pindel, E.V.; Kedishvili, N.Y.; Abraham, T.L.; Brzezinski, M.R.; Zhang, J.; Dean, R.A.; Bosron, W.F.; J. Biol. Chem. 272, 14769-14775 (1997)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
-
Homo sapiens
Inhibitors
Inhibitors
Commentary
Organism
Structure
eserine
hCE-2 shows greater inhibition by eserine thann hCE-1
Homo sapiens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.13
-
6-monoacetylmorphine
pH 7.4, 37C
Homo sapiens
0.15
-
4-yethylumbelliferyl acetate
pH 7.4, 37C
Homo sapiens
0.39
-
cocaine
pH 7.4, 37C
Homo sapiens
6.8
-
heroin
pH 7.4, 37C
Homo sapiens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
60000
-
1 * 60000, SDS-PAGE
Homo sapiens
80000
-
gel filtration
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
cocaine + H2O
Homo sapiens
hCE-2 exhibits different drug ester substrate specificity from the human liver carboxylesterase hCE-1, which hydrolyzes the methyl ester of cocaine. hCE-2 may play an important role in the degradation of cocaine and heroin in human tissues
ecgonine methyl ester + benzoate
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Homo sapiens
O00748
-
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
glycoprotein
high mannose type
Homo sapiens
Purification (Commentary)
Purification (Commentary)
Organism
-
Homo sapiens
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Homo sapiens
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
4-methylumbelliferyl acetate + H2O
hCE-2 has higher catalytic efficiency for hydrolysis than hCE-1
171019
Homo sapiens
4-methylumbelliferone + acetate
-
-
-
?
6-monoacetylmorphine + H2O
hCE-2 has higher catalytic efficiency for hydrolysis than hCE-1
171019
Homo sapiens
morphine + acetate
-
-
-
?
cocaine + H2O
hCE-2 exhibits different drug ester substrate specificity from the human liver carboxylesterase hCE-1, which hydrolyzes the methyl ester of cocaine. hCE-2 may play an important role in the degradation of cocaine and heroin in human tissues
171019
Homo sapiens
ecgonine methyl ester + benzoate
-
-
-
?
cocaine + H2O
hCE-2 exhibits different drug ester substrate specificity from the human liver carboxylesterase hCE-1, which hydrolyzes the methyl ester of cocaine
171019
Homo sapiens
ecgonine methyl ester + benzoate
-
-
-
?
heroin + H2O
hCE-2 has higher catalytic efficiency for hydrolysis than hCE-1
171019
Homo sapiens
6-monoacetylmorphine + acetate
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 60000, SDS-PAGE
Homo sapiens
Synonyms
Synonyms
Commentary
Organism
hCE-2
-
Homo sapiens
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0001
-
eserine
-
Homo sapiens
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Homo sapiens
isoelectric focusing
-
4.9
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Homo sapiens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
eserine
hCE-2 shows greater inhibition by eserine thann hCE-1
Homo sapiens
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.0001
-
eserine
-
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.13
-
6-monoacetylmorphine
pH 7.4, 37C
Homo sapiens
0.15
-
4-yethylumbelliferyl acetate
pH 7.4, 37C
Homo sapiens
0.39
-
cocaine
pH 7.4, 37C
Homo sapiens
6.8
-
heroin
pH 7.4, 37C
Homo sapiens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
60000
-
1 * 60000, SDS-PAGE
Homo sapiens
80000
-
gel filtration
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
cocaine + H2O
Homo sapiens
hCE-2 exhibits different drug ester substrate specificity from the human liver carboxylesterase hCE-1, which hydrolyzes the methyl ester of cocaine. hCE-2 may play an important role in the degradation of cocaine and heroin in human tissues
ecgonine methyl ester + benzoate
-
-
?
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
glycoprotein
high mannose type
Homo sapiens
Purification (Commentary) (protein specific)
Commentary
Organism
-
Homo sapiens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
4-methylumbelliferyl acetate + H2O
hCE-2 has higher catalytic efficiency for hydrolysis than hCE-1
171019
Homo sapiens
4-methylumbelliferone + acetate
-
-
-
?
6-monoacetylmorphine + H2O
hCE-2 has higher catalytic efficiency for hydrolysis than hCE-1
171019
Homo sapiens
morphine + acetate
-
-
-
?
cocaine + H2O
hCE-2 exhibits different drug ester substrate specificity from the human liver carboxylesterase hCE-1, which hydrolyzes the methyl ester of cocaine. hCE-2 may play an important role in the degradation of cocaine and heroin in human tissues
171019
Homo sapiens
ecgonine methyl ester + benzoate
-
-
-
?
cocaine + H2O
hCE-2 exhibits different drug ester substrate specificity from the human liver carboxylesterase hCE-1, which hydrolyzes the methyl ester of cocaine
171019
Homo sapiens
ecgonine methyl ester + benzoate
-
-
-
?
heroin + H2O
hCE-2 has higher catalytic efficiency for hydrolysis than hCE-1
171019
Homo sapiens
6-monoacetylmorphine + acetate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 60000, SDS-PAGE
Homo sapiens
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Homo sapiens
isoelectric focusing
-
4.9
Other publictions for EC 3.1.1.84
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
750291
Kim
Kinetic characterization of c ...
Homo sapiens
Chem. Biol. Interact.
293
107-114
2018
-
-
1
-
-
-
1
3
-
-
-
3
-
1
-
-
-
-
-
-
-
-
5
-
4
1
-
-
3
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
1
3
-
-
-
3
-
-
-
-
-
-
-
-
5
-
1
-
-
3
1
-
-
-
-
1
1
-
3
3
728856
Fang
Rational design, preparation, ...
Rhodococcus sp., Rhodococcus sp. MB1 Bresler
ACS Chem. Biol.
9
1764-1772
2014
-
-
1
1
3
-
-
3
-
-
-
2
-
3
-
-
1
-
-
-
-
-
2
1
1
-
-
-
3
-
-
-
-
-
-
-
-
-
1
-
1
3
-
-
-
-
3
-
-
-
2
-
-
-
1
-
-
-
-
2
1
-
-
-
3
-
-
-
-
-
-
-
-
3
3
730250
Brim
The fate of bacterial cocaine ...
Rhodococcus sp.
J. Pharmacol. Exp. Ther.
340
83-95
2012
-
-
1
-
-
-
-
-
-
-
1
1
-
4
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
730421
Lee
Cell permeable cocaine esteras ...
Rhodococcus sp., Rhodococcus sp. MB1
Mol. Pharm.
9
1361-1373
2012
-
-
1
-
5
-
-
6
-
-
1
2
-
6
-
-
1
-
-
-
-
-
2
1
1
-
-
1
6
-
-
-
-
-
-
-
-
-
1
-
-
5
-
-
-
-
6
-
-
1
2
-
-
-
1
-
-
-
-
2
1
-
-
1
6
-
-
-
-
-
-
-
-
6
6
716278
Xue
Design, preparation, and chara ...
Homo sapiens
Mol. Pharmacol.
79
290-297
2011
-
-
1
-
3
-
-
4
-
-
-
-
-
1
-
-
1
-
-
1
-
-
1
-
2
-
-
-
4
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
4
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
4
4
716281
Narasimhan
Subunit stabilization and pegy ...
Rhodococcus sp.
Mol. Pharmacol.
80
1056-1065
2011
-
-
1
1
3
-
-
4
-
-
1
-
-
3
-
-
1
-
-
-
-
-
1
1
1
-
-
1
4
-
-
-
-
-
-
-
-
-
1
-
1
3
-
-
-
-
4
-
-
1
-
-
-
-
1
-
-
-
-
1
1
-
-
1
4
-
-
-
-
-
-
-
-
4
4
714226
Zheng
Design of high-activity mutant ...
Homo sapiens
Biochemistry
49
9113-9119
2010
-
-
1
1
10
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
9
9
714702
Yang
Characterization of a high-act ...
Homo sapiens
Chem. Biol. Interact.
187
148-152
2010
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
2
2
716276
Brim
A thermally stable form of bac ...
Rhodococcus sp., Rhodococcus sp. MB1
Mol. Pharmacol.
77
593-600
2010
-
1
-
1
1
-
-
4
-
-
-
-
-
7
-
-
-
-
-
-
-
-
2
-
1
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
1
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
716824
Narasimhan
Structural analysis of thermos ...
Rhodococcus sp., Rhodococcus sp. MB1
Protein Eng.
23
537-547
2010
-
1
1
-
5
-
-
4
-
-
-
-
-
6
-
-
1
-
-
-
-
-
2
-
1
-
-
1
5
-
-
-
-
-
-
-
-
1
1
-
-
5
-
-
-
-
4
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
1
5
-
-
-
-
-
-
-
-
5
5
692028
Ko
Effects of cocaine esterase fo ...
Rhodococcus sp.
Drug Alcohol Depend.
101
202-209
2009
-
1
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
694267
Gao
Thermostable variants of cocai ...
Rhodococcus sp.
Mol. Pharmacol.
75
318-323
2009
-
1
-
-
4
-
-
3
-
-
-
1
-
1
-
-
1
-
-
-
-
-
2
-
1
1
-
1
4
1
-
-
-
-
-
-
-
1
-
-
-
4
-
-
-
-
3
-
-
-
1
-
-
-
1
-
-
-
-
2
-
1
-
1
4
1
-
-
-
-
-
-
-
-
-
699760
Collins
Zhan. C.G.; Woods. J.H.: Cocai ...
Rhodococcus sp.
J. Pharmacol. Exp. Ther.
331
445-455
2009
-
1
-
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
698483
Liu
Fundamental reaction mechanism ...
Rhodococcus sp.
J. Am. Chem. Soc.
131
11964-11975
2003
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
650108
Turner
Biochemical characterization a ...
Rhodococcus sp.
Biochemistry
41
12297-12307
2002
-
1
1
1
11
-
-
10
-
-
-
1
1
2
-
-
-
-
-
-
-
-
3
-
1
-
-
-
9
1
1
-
-
-
-
-
-
1
1
-
1
11
-
-
-
-
10
-
-
-
1
1
-
-
-
-
-
-
-
3
-
-
-
-
9
1
1
-
-
-
-
-
-
-
-
653322
Larsen
Crystal structure of a bacteri ...
Rhodococcus sp.
Nat. Struct. Biol.
9
17-21
2002
-
1
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
695729
Bresler
Gene cloning and nucleotide se ...
Rhodococcus sp., Rhodococcus sp. MB1
Appl. Environ. Microbiol.
66
904-908
2000
-
-
1
-
-
-
-
1
-
-
2
2
-
11
-
-
1
-
-
1
1
-
6
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
2
2
-
-
-
1
-
1
1
-
6
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
171019
Pindel
Purification and cloning of a ...
Homo sapiens
J. Biol. Chem.
272
14769-14775
1997
-
-
1
-
-
-
1
4
-
-
2
1
-
1
-
1
1
-
-
1
-
-
5
1
1
-
-
-
-
-
-
-
-
1
1
-
-
-
1
-
-
-
-
-
1
1
4
-
-
2
1
-
-
1
1
-
1
-
-
5
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
698540
Britt
Identification of a cocaine es ...
Stenotrophomonas maltophilia, Stenotrophomonas maltophilia MB11L
J. Bacteriol.
174
2087-2094
1992
-
-
-
-
-
-
2
3
-
-
3
2
-
4
-
-
1
-
-
-
1
-
10
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
3
-
-
3
2
-
-
-
1
-
-
1
-
10
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-