Cloned (Comment) | Organism |
---|---|
recombinant PPD is produced using a glutathione S-transferase (GST) fusion system. The PPD and GST genes are fused to a pGEX-2T vector and expressed in Escherichia coli BL21(DE3)pLysS strain under the control of a T7 promoter as a fusion protein | Raphanus sativus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.955 | - |
pheophorbide a | PPD-GST has about 6fold lower affinity for Pheid a than native PPDs | Raphanus sativus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
PPD-GST fusion protein, SDS-PAGE | Raphanus sativus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pheophorbide a + H2O | Raphanus sativus | - |
pyropheophorbide a + methanol + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Raphanus sativus | Q2V0W1 | - |
- |
Purification (Comment) | Organism |
---|---|
single-step affinity chromatography through a GSTrap FF column | Raphanus sativus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cotyledon | - |
Raphanus sativus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pheophorbide a + H2O | - |
Raphanus sativus | pyropheophorbide a + methanol + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
pheophorbidase | - |
Raphanus sativus |
Ppd | - |
Raphanus sativus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 80 | - |
Raphanus sativus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
50% loss of activity | Raphanus sativus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | 60 mM MES-HEPES-tricine buffer is used as an incubation medium in the pH range of 5.5 to 9.0 | Raphanus sativus |