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Literature summary for 3.1.1.81 extracted from

  • Jacquet, P.; Hiblot, J.; Daude, D.; Bergonzi, C.; Gotthard, G.; Armstrong, N.; Chabriere, E.; Elias, M.
    Rational engineering of a native hyperthermostable lactonase into a broad spectrum phosphotriesterase (2017), Sci. Rep., 7, 16745 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.81

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Saccharolobus solfataricus

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 20-30% (w/v) PEG 8000 and 50 mM Tris-HCl buffer (pH 8.0) Saccharolobus solfataricus

Protein Variants

Protein Variants Comment Organism
C258L/I261F/W263A the mutant exhibits enhanced phosphotriesterase activity compared to the wild type enzyme Saccharolobus solfataricus
F46L/C258A/W263M/I280T the mutant exhibits enhanced phosphotriesterase activity compared to the wild type enzyme Saccharolobus solfataricus
L72I/Y99F/I122L/L228M/F229S/W263L the mutant exhibits enhanced phosphotriesterase activity compared to the wild type enzyme Saccharolobus solfataricus
V27A/I76T/Y97W/Y99F/L130P/L226V the mutant exhibits enhanced phosphotriesterase activity compared to the wild type enzyme Saccharolobus solfataricus
V27A/Y97W/L228M/W263M the mutant demonstrates a large increase in catalytic efficiencies over the wild-type enzyme, with increases of 2210fold, 163fold, 58fold, 16fold against methyl parathion, malathion, ethyl paraoxon, and methyl paraoxon, respectively Saccharolobus solfataricus

Inhibitors

Inhibitors Comment Organism Structure
fensulfothion
-
 Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
an N-acyl-L-homoserine lactone + H2O Saccharolobus solfataricus
-
 an N-acyl-L-homoserine
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q97VT7 Sulfolobus solfataricus
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and Superdex 75 gel filtration Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
an N-acyl-L-homoserine lactone + H2O
-
 Saccharolobus solfataricus an N-acyl-L-homoserine
-
 ?
additional information no activity with coumaphos, fensulfothion, fenitrothion, diazinon, chlorpyrifos, and ethyl parathion. Low activity with malathion, methyl parathion methyl paraoxon, and ethyl paraoxon Saccharolobus solfataricus ?
-
 ?
N-3-oxododecanoyl-L-homoserine lactone + H2O
-
 Saccharolobus solfataricus N-3-oxododecanoyl-L-homoserine
-
 ?
undecanoic-delta-lactone + H2O
-
 Saccharolobus solfataricus 5-hydroxyundecanoic acid
-
 ?
undecanoic-gamma-lactone + H2O
-
 Saccharolobus solfataricus 4-hydroxyundecanoic acid
-
 ?

Synonyms

Synonyms Comment Organism
POX
-
 Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.141
-
 N-3-oxododecanoyl-L-homoserine lactone mutant enzyme C258L/I261F/W263A, at pH 8.0 and 25°C Saccharolobus solfataricus
0.584
-
 N-3-oxododecanoyl-L-homoserine lactone mutant enzyme L72I/Y99F/I122L/L228M/F229S/W263L, at pH 8.0 and 25°C Saccharolobus solfataricus
0.905
-
 undecanoic-gamma-lactone mutant enzyme V27A/I76T/Y97W/Y99F/L130P/L226V, at pH 8.0 and 25°C Saccharolobus solfataricus
1.63
-
 undecanoic-gamma-lactone mutant enzyme C258L/I261F/W263A, at pH 8.0 and 25°C Saccharolobus solfataricus
1.78
-
 N-3-oxododecanoyl-L-homoserine lactone mutant enzyme F46L/C258A/W263M/I280T, at pH 8.0 and 25°C Saccharolobus solfataricus
2.36
-
 undecanoic-gamma-lactone wild type enzyme, at pH 8.0 and 25°C Saccharolobus solfataricus
2.55
-
 undecanoic-delta-lactone wild type enzyme, at pH 8.0 and 25°C Saccharolobus solfataricus
2.55
-
 undecanoic-delta-lactone mutant enzyme C258L/I261F/W263A, at pH 8.0 and 25°C Saccharolobus solfataricus
5.33
-
 undecanoic-delta-lactone mutant enzyme V27A/I76T/Y97W/Y99F/L130P/L226V, at pH 8.0 and 25°C Saccharolobus solfataricus
7.5
-
 undecanoic-delta-lactone mutant enzyme V27A/Y97W/L228M/W263M, at pH 8.0 and 25°C Saccharolobus solfataricus
16
-
 undecanoic-gamma-lactone mutant enzyme V27A/Y97W/L228M/W263M, at pH 8.0 and 25°C Saccharolobus solfataricus
31.6
-
 N-3-oxododecanoyl-L-homoserine lactone wild type enzyme, at pH 8.0 and 25°C Saccharolobus solfataricus
360
-
 undecanoic-delta-lactone mutant enzyme L72I/Y99F/I122L/L228M/F229S/W263L, at pH 8.0 and 25°C Saccharolobus solfataricus
440
-
 undecanoic-gamma-lactone mutant enzyme F46L/C258A/W263M/I280T, at pH 8.0 and 25°C Saccharolobus solfataricus
573
-
 undecanoic-gamma-lactone mutant enzyme L72I/Y99F/I122L/L228M/F229S/W263L, at pH 8.0 and 25°C Saccharolobus solfataricus
9650
-
 undecanoic-delta-lactone mutant enzyme F46L/C258A/W263M/I280T, at pH 8.0 and 25°C Saccharolobus solfataricus