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Literature summary for 3.1.1.81 extracted from

  • Morohoshi, T.; Tominaga, Y.; Someya, N.; Ikeda, T.
    Characterization of a novel thermostable N-acylhomoserine lactonase from the thermophilic bacterium Thermaerobacter marianensis (2015), J. Biosci. Bioeng., 120, 1-5 .
    View publication on PubMed
Search for more literature for 3.1.1.81

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus cereus
expression in Escherichia coli Thermaerobacter marianensis
expression in Escherichia coli Chryseobacterium sp. StRB126
expression in Escherichia coli Thermaerobacter nagasakiensis
expression in Escherichia coli Thermaerobacter composti
expression in Escherichia coli Solibacillus silvestris

Organism

Organism UniProt Comment Textmining
Bacillus cereus
-
-
-
Bacillus cereus ATCC 14579
-
-
-
Chryseobacterium sp. StRB126 I7HB71
-
-
Solibacillus silvestris F8WSM9
-
-
Solibacillus silvestris StLB046 F8WSM9
-
-
Thermaerobacter composti A0A060NTE1
-
-
Thermaerobacter composti JCM 15650 A0A060NTE1
-
-
Thermaerobacter marianensis
-
-
-
Thermaerobacter marianensis JCM 10246
-
-
-
Thermaerobacter nagasakiensis A0A060NR36
-
-
Thermaerobacter nagasakiensis JCM 11223 A0A060NR36
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant maltose binding protein (MBP)-AiiT fusion protein Bacillus cereus
recombinant maltose binding protein (MBP)-AiiT fusion protein Thermaerobacter marianensis
recombinant maltose binding protein (MBP)-AiiT fusion protein Chryseobacterium sp. StRB126
recombinant maltose binding protein (MBP)-AiiT fusion protein Thermaerobacter nagasakiensis
recombinant maltose binding protein (MBP)-AiiT fusion protein Thermaerobacter composti
recombinant maltose binding protein (MBP)-AiiT fusion protein Solibacillus silvestris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-decanoyl-L-homoserine lactone + H2O
-
 Thermaerobacter marianensis N-decanoyl-L-homoserine
-
 ?
N-decanoyl-L-homoserine lactone + H2O
-
 Thermaerobacter marianensis JCM 10246 N-decanoyl-L-homoserine
-
 ?
N-hexanoyl-L-homoserine lactone + H2O
-
 Thermaerobacter marianensis N-hexanoyl-L-homoserine
-
 ?
N-hexanoyl-L-homoserine lactone + H2O
-
 Thermaerobacter marianensis JCM 10246 N-hexanoyl-L-homoserine
-
 ?
N-octanoyl-L-homoserine lactone + H2O
-
 Thermaerobacter marianensis N-octanoyl-L-homoserine
-
 ?
N-octanoyl-L-homoserine lactone + H2O
-
 Thermaerobacter marianensis JCM 10246 N-octanoyl-L-homoserine
-
 ?

Synonyms

Synonyms Comment Organism
AhlS
-
 Solibacillus silvestris
AidC
-
 Chryseobacterium sp. StRB126
AiiA
-
 Bacillus cereus
AiiT
-
 Thermaerobacter marianensis
AiiT
-
 Thermaerobacter nagasakiensis
AiiT
-
 Thermaerobacter composti

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 40
-
 Bacillus cereus
30 40
-
 Chryseobacterium sp. StRB126
50 60
-
 Solibacillus silvestris
60 80
-
 Thermaerobacter marianensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 60 20°C: about 85% of maximal activity, 60°C: about 60% of maximal activity Chryseobacterium sp. StRB126
20 60 20°C: about 90% of maximal activity, 60°C: about 50% of maximal activity Bacillus cereus
20 70 20°C: about 35% of maximal activity, 70°C: about 50% of maximal activity Solibacillus silvestris
40 90 40°C: about 70% of maximal activity, 90°C: about 50% of maximal activity Thermaerobacter marianensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 10 min, AHL lactonase activity is almost completely lost Bacillus cereus
40
-
 10 min, AHL lactonase activity is almost completely lost Chryseobacterium sp. StRB126
40
-
 10 min, AHL lactonase activity is almost completely lost Solibacillus silvestris
40
-
 10 min, over 80% of the AHL-lactonase activity of MBP-AiiT is retained Thermaerobacter marianensis
60
-
 10 min, AHL lactonase activity is almost completely lost Bacillus cereus
60
-
 10 min, AHL lactonase activity is almost completely lost Chryseobacterium sp. StRB126
60
-
 10 min, AHL lactonase activity is almost completely lost Solibacillus silvestris
60
-
 10 min, over 80% of the AHL-lactonase activity of MBP-AiiT is retained Thermaerobacter marianensis
70
-
 10 min, AHL lactonase activity is almost completely lost Bacillus cereus
70
-
 10 min, AHL lactonase activity is almost completely lost Chryseobacterium sp. StRB126
70
-
 10 min, AHL lactonase activity is almost completely lost Solibacillus silvestris
70
-
 10 min, over 80% of the AHL-lactonase activity of MBP-AiiT is retained Thermaerobacter marianensis
90
-
 10 min, approximately 50% activity is retained Thermaerobacter marianensis