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Literature summary for 3.1.1.81 extracted from

  • Tang, K.; Su, Y.; Brackman, G.; Cui, F.; Zhang, Y.; Shi, X.; Coenye, T.; Zhang, X.
    MomL, a novel marine-derived N-Acyl homoserine lactonase from Muricauda olearia (2015), Appl. Environ. Microbiol., 81, 774-782 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.81

Application

Application Comment Organism
drug development MomL significantly attenuates the virulence of Pseudomonas aeruginosa in a Caenorhabditis elegans infection model, which suggests that MomL has the potential to be used as a therapeutic agent Muricauda olearia
pharmacology MomL significantly attenuates the virulence of Pseudomonas aeruginosa in a Caenorhabditis elegans infection model, which suggests that MomL has the potential to be used as a therapeutic agent Muricauda olearia

Cloned(Commentary)

Cloned (Comment) Organism
Escherichia coli BL21(DE3) Muricauda olearia

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 0.1 mM, complete inhibition Muricauda olearia
EDTA
-
 Muricauda olearia
Fe2+ 0.1 mM, about 80% inhibition Muricauda olearia
Ni2+ 0.1 mM, about 40% inhibition Muricauda olearia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.44
-
 N-octanoyl-L-homoserine lactone pH 7.1, 25°C Muricauda olearia
0.44
-
 N-3-oxododecanoyl-L-homoserine lactone pH 7.1, 25°C Muricauda olearia
0.49
-
 N-3-oxooctanoyl-L-homoserine lactone pH 7.1, 25°C Muricauda olearia
0.79
-
 N-hexanoyl-L-homoserine lactone pH 7.1, 25°C Muricauda olearia
0.85
-
 N-butyryl-L-homoserine lactone pH 7.1, 25°C Muricauda olearia
0.95
-
 N-3-oxohexanoyl-L-homoserine lactone pH 7.1, 25°C Muricauda olearia

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Muricauda olearia
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 0.1 mM, 1.4fold activation Muricauda olearia
Co2+ 0.1 mM, 1.5fold activation Muricauda olearia
Mg2+ 0.1 mM, 1.5fold activation. The purified MomL contains 0.57 equivalents of magnesium when the medium is not supplemented with extra metal ions. When the growth medium is supplemented with ZnCl2 (0.2 mM), the molar ratios of purified MomL to zinc and magnesium are determined to be 1.67 and 0.23 equivalents Muricauda olearia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
 purified recombinant enzyme Muricauda olearia

Organism

Organism UniProt Comment Textmining
Muricauda olearia A0A0A1EIU0
-
-
Muricauda olearia Th120 A0A0A1EIU0
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Muricauda olearia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-3-oxododecanoyl-L-homoserine lactone + H2O
-
 Muricauda olearia N-3-oxododecanoyl-L-homoserine
-
 ?
N-3-oxododecanoyl-L-homoserine lactone + H2O
-
 Muricauda olearia Th120 N-3-oxododecanoyl-L-homoserine
-
 ?
N-3-oxohexanoyl-L-homoserine lactone + H2O
-
 Muricauda olearia N-3-oxohexanoyl-L-homoserine
-
 ?
N-3-oxohexanoyl-L-homoserine lactone + H2O
-
 Muricauda olearia Th120 N-3-oxohexanoyl-L-homoserine
-
 ?
N-3-oxooctanoyl-L-homoserine lactone + H2O
-
 Muricauda olearia N-3-oxooctanoyl-L-homoserine
-
 ?
N-3-oxooctanoyl-L-homoserine lactone + H2O
-
 Muricauda olearia Th120 N-3-oxooctanoyl-L-homoserine
-
 ?
N-butyryl-L-homoserine lactone + H2O
-
 Muricauda olearia N-butyryl-L-homoserine
-
 ?
N-dodecanoyl-L-homoserine lactone + H2O
-
 Muricauda olearia N-dodecanoyl-L-homoserine
-
 ?
N-hexanoyl-L-homoserine lactone + H2O
-
 Muricauda olearia N-hexanoyl-L-homoserine
-
 ?
N-hexanoyl-L-homoserine lactone + H2O
-
 Muricauda olearia Th120 N-hexanoyl-L-homoserine
-
 ?
N-octanoyl-L-homoserine lactone + H2O
-
 Muricauda olearia N-octanoyl-L-homoserine
-
 ?
N-octanoyl-L-homoserine lactone + H2O
-
 Muricauda olearia Th120 N-octanoyl-L-homoserine
-
 ?

Synonyms

Synonyms Comment Organism
AHL lactonase
-
 Muricauda olearia
MomL
-
 Muricauda olearia

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Muricauda olearia

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 60 10°C: about 30% of maximal activity, 20°C: about 65% of maximal activity, 50°C: about 90% of maximal activity, 60°C: about 15% of maximal activity Muricauda olearia

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 30 min, no loss of activity Muricauda olearia
60
-
 30 min, the enzyme retains about 30% of its activity Muricauda olearia
100
-
 30 min, the enzyme retains about 10% of its activity Muricauda olearia

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
135
-
 N-butyryl-L-homoserine lactone pH 7.1, 25°C Muricauda olearia
158
-
 N-octanoyl-L-homoserine lactone pH 7.1, 25°C Muricauda olearia
218
-
 N-3-oxooctanoyl-L-homoserine lactone pH 7.1, 25°C Muricauda olearia
224
-
 N-3-oxododecanoyl-L-homoserine lactone pH 7.1, 25°C Muricauda olearia
226
-
 N-hexanoyl-L-homoserine lactone pH 7.1, 25°C Muricauda olearia
293
-
 N-3-oxohexanoyl-L-homoserine lactone pH 7.1, 25°C Muricauda olearia

pH Stability

pH Stability pH Stability Maximum Comment Organism
3
-
 3 h, about 80% loss of activity Muricauda olearia
5
-
 3 h, 75% loss of activity Muricauda olearia
6
-
 3 h, 65% loss of activity Muricauda olearia
7
-
 3 h, 10% loss of activity Muricauda olearia
8
-
 3 h, high stability Muricauda olearia
9 11 3 h, 10% loss of activity Muricauda olearia

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
160
-
 N-butyryl-L-homoserine lactone pH 7.1, 25°C Muricauda olearia
290
-
 N-hexanoyl-L-homoserine lactone pH 7.1, 25°C Muricauda olearia
310
-
 N-3-oxohexanoyl-L-homoserine lactone pH 7.1, 25°C Muricauda olearia
360
-
 N-octanoyl-L-homoserine lactone pH 7.1, 25°C Muricauda olearia
450
-
 N-3-oxooctanoyl-L-homoserine lactone pH 7.1, 25°C Muricauda olearia
510
-
 N-3-oxododecanoyl-L-homoserine lactone pH 7.1, 25°C Muricauda olearia