Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.81 extracted from

  • Bergonzi, C.; Schwab, M.; Elias, M.
    The quorum-quenching lactonase from Geobacillus caldoxylosilyticus purification, characterization, crystallization and crystallographic analysis (2016), Acta Crystallogr. Sect. F, 72, 681-686 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.81

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain BL21 (DE3)-pGro7/GroEL Parageobacillus caldoxylosilyticus

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting-drop vapor-diffusion method, X-ray diffraction data collection to 1.6 A resolution Parageobacillus caldoxylosilyticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00145
-
 N-decanoyl-L-homoserine lactone pH 8.3, 25°C Parageobacillus caldoxylosilyticus
0.00219
-
 N-3-oxododecanoyl-L-homoserine lactone pH 8.3, 25°C Parageobacillus caldoxylosilyticus
0.00797
-
 N-hexanoyl-L-homoserine lactone pH 8.3, 25°C Parageobacillus caldoxylosilyticus
0.229
-
 N-butyryl-L-homoserine lactone pH 8.3, 25°C Parageobacillus caldoxylosilyticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 SDS-PAGE Parageobacillus caldoxylosilyticus

Organism

Organism UniProt Comment Textmining
Parageobacillus caldoxylosilyticus A0A023DFE8
-
-
Parageobacillus caldoxylosilyticus NBRC 107762 A0A023DFE8
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Parageobacillus caldoxylosilyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-3-oxododecanoyl-L-homoserine lactone + H2O
-
 Parageobacillus caldoxylosilyticus N-3-oxododecanoyl-L-homoserine
-
 ?
N-3-oxododecanoyl-L-homoserine lactone + H2O
-
 Parageobacillus caldoxylosilyticus NBRC 107762 N-3-oxododecanoyl-L-homoserine
-
 ?
N-butyryl-L-homoserine lactone + H2O
-
 Parageobacillus caldoxylosilyticus N-butyryl-L-homoserine
-
 ?
N-butyryl-L-homoserine lactone + H2O
-
 Parageobacillus caldoxylosilyticus NBRC 107762 N-butyryl-L-homoserine
-
 ?
N-decanoyl-L-homoserine lactone + H2O
-
 Parageobacillus caldoxylosilyticus N-decanoyl-L-homoserine
-
 ?
N-decanoyl-L-homoserine lactone + H2O
-
 Parageobacillus caldoxylosilyticus NBRC 107762 N-decanoyl-L-homoserine
-
 ?
N-hexanoyl-L-homoserine lactone + H2O
-
 Parageobacillus caldoxylosilyticus N-hexanoyl-L-homoserine
-
 ?
N-hexanoyl-L-homoserine lactone + H2O
-
 Parageobacillus caldoxylosilyticus NBRC 107762 N-hexanoyl-L-homoserine
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 32000, SDS-PAGE Parageobacillus caldoxylosilyticus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
75
-
 half-life: 152.5 min Parageobacillus caldoxylosilyticus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.48
-
 N-decanoyl-L-homoserine lactone pH 8.3, 25°C Parageobacillus caldoxylosilyticus
8.95
-
 N-hexanoyl-L-homoserine lactone pH 8.3, 25°C Parageobacillus caldoxylosilyticus
9.48
-
 N-3-oxododecanoyl-L-homoserine lactone pH 8.3, 25°C Parageobacillus caldoxylosilyticus
19.06
-
 N-butyryl-L-homoserine lactone pH 8.3, 25°C Parageobacillus caldoxylosilyticus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
83.1
-
 N-butyryl-L-homoserine lactone pH 8.3, 25°C Parageobacillus caldoxylosilyticus
1123
-
 N-hexanoyl-L-homoserine lactone pH 8.3, 25°C Parageobacillus caldoxylosilyticus
3770
-
 N-decanoyl-L-homoserine lactone pH 8.3, 25°C Parageobacillus caldoxylosilyticus
4310
-
 N-3-oxododecanoyl-L-homoserine lactone pH 8.3, 25°C Parageobacillus caldoxylosilyticus