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Literature summary for 3.1.1.81 extracted from

  • Park, S.Y.; Lee, S.J.; Oh, T.K.; Oh, J.W.; Koo, B.T.; Yum, D.Y.; Lee, J.K.
    AhlD, an N-acylhomoserine lactonase in Arthrobacter sp., and predicted homologues in other bacteria (2003), Microbiology, 149, 1541-1550.
    View publication on PubMed
Search for more literature for 3.1.1.81

Cloned(Commentary)

Cloned (Comment) Organism
gene ahlD, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strains DH5alpha, BL21(DE3) as His-tagged enzyme, and JM109 Arthrobacter sp.
gene ahlK, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain JM109 Klebsiella pneumoniae

General Stability

General Stability Organism
the enzyme is instable during purification Arthrobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 x * 30000, native enzyme, SDS-PAGE, x * 36000, recombinant enzyme, SDS-PAGE Arthrobacter sp.
36000
-
 x * 30000, native enzyme, SDS-PAGE, x * 36000, recombinant enzyme, SDS-PAGE Arthrobacter sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Arthrobacter sp. quorum-sensing is a signaling mechanism, that controls diverse biological functions, including virulence, via N-acylhomoserine lactone signal molecules in Gram-negative bacteria, overview ?
-
 ?
additional information Klebsiella pneumoniae quorum-sensing is a signaling mechanism, that controls diverse biological functions, including virulence, via N-acylhomoserine lactone signal molecules in Gram-negative bacteria, overview ?
-
 ?
additional information Arthrobacter sp. IBN110 quorum-sensing is a signaling mechanism, that controls diverse biological functions, including virulence, via N-acylhomoserine lactone signal molecules in Gram-negative bacteria, overview ?
-
 ?
additional information Klebsiella pneumoniae KCTC 2241 / ATCC 25306 quorum-sensing is a signaling mechanism, that controls diverse biological functions, including virulence, via N-acylhomoserine lactone signal molecules in Gram-negative bacteria, overview ?
-
 ?
N-acyl-(S)-homoserine lactone + H2O Arthrobacter sp. the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora strain N98 N-acyl-(S)-homoserine
-
 ?
N-acyl-(S)-homoserine lactone + H2O Klebsiella pneumoniae the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of pathogenic bacteria N-acyl-(S)-homoserine
-
 ?
N-acyl-(S)-homoserine lactone + H2O Arthrobacter sp. IBN110 the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora strain N98 N-acyl-(S)-homoserine
-
 ?
N-acyl-(S)-homoserine lactone + H2O Klebsiella pneumoniae KCTC 2241 / ATCC 25306 the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of pathogenic bacteria N-acyl-(S)-homoserine
-
 ?

Organism

Organism UniProt Comment Textmining
Arthrobacter sp. Q7X3T2 gene ahlD
-
Arthrobacter sp. IBN110 Q7X3T2 gene ahlD
-
Klebsiella pneumoniae Q7X477 gene ahlK
-
Klebsiella pneumoniae KCTC 2241 / ATCC 25306 Q7X477 gene ahlK
-

Purification (Commentary)

Purification (Comment) Organism
partially from strain IBN110 by ammonium sulfate fractionation and two steps of ion exchange chromatography, full purification failed due to enzyme instability Arthrobacter sp.

Reaction

Reaction Comment Organism Reaction ID
an N-acyl-L-homoserine lactone + H2O = an N-acyl-L-homoserine hydrolysis of the lactone ring Arthrobacter sp.
a
an N-acyl-L-homoserine lactone + H2O = an N-acyl-L-homoserine hydrolysis of the lactone ring Klebsiella pneumoniae
a

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:N-3-oxododecanoyl-L-homoserine lactone/ammonium chloride-grown cell
-
 Arthrobacter sp.
-
culture condition:N-3-oxododecanoyl-L-homoserine lactone/ammonium chloride-grown cell
-
 Klebsiella pneumoniae
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Arthrobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information quorum-sensing is a signaling mechanism, that controls diverse biological functions, including virulence, via N-acylhomoserine lactone signal molecules in Gram-negative bacteria, overview Arthrobacter sp. ?
-
 ?
additional information quorum-sensing is a signaling mechanism, that controls diverse biological functions, including virulence, via N-acylhomoserine lactone signal molecules in Gram-negative bacteria, overview Klebsiella pneumoniae ?
-
 ?
additional information quorum-sensing is a signaling mechanism, that controls diverse biological functions, including virulence, via N-acylhomoserine lactone signal molecules in Gram-negative bacteria, overview Arthrobacter sp. IBN110 ?
-
 ?
additional information quorum-sensing is a signaling mechanism, that controls diverse biological functions, including virulence, via N-acylhomoserine lactone signal molecules in Gram-negative bacteria, overview Klebsiella pneumoniae KCTC 2241 / ATCC 25306 ?
-
 ?
N-3-oxododecanoyl-L-homoserine lactone + H2O
-
 Arthrobacter sp. N-3-oxododecanoyl-L-homoserine
-
 ?
N-3-oxododecanoyl-L-homoserine lactone + H2O
-
 Arthrobacter sp. IBN110 N-3-oxododecanoyl-L-homoserine
-
 ?
N-3-oxohexanoyl-L-homoserine lactone + H2O
-
 Arthrobacter sp. N-3-oxohexanoyl-L-homoserine
-
 ?
N-3-oxohexanoyl-L-homoserine lactone + H2O
-
 Klebsiella pneumoniae N-3-oxohexanoyl-L-homoserine
-
 ?
N-3-oxohexanoyl-L-homoserine lactone + H2O
-
 Klebsiella pneumoniae KCTC 2241 / ATCC 25306 N-3-oxohexanoyl-L-homoserine
-
 ?
N-acyl-(S)-homoserine lactone + H2O the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora strain N98 Arthrobacter sp. N-acyl-(S)-homoserine
-
 ?
N-acyl-(S)-homoserine lactone + H2O the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of pathogenic bacteria Klebsiella pneumoniae N-acyl-(S)-homoserine
-
 ?
N-acyl-(S)-homoserine lactone + H2O the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora strain N98 Arthrobacter sp. IBN110 N-acyl-(S)-homoserine
-
 ?
N-acyl-(S)-homoserine lactone + H2O the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of pathogenic bacteria Klebsiella pneumoniae KCTC 2241 / ATCC 25306 N-acyl-(S)-homoserine
-
 ?
N-butanoyl-L-homoserine lactone + H2O
-
 Arthrobacter sp. N-butanoyl-L-homoserine
-
 ?
N-decanoyl-L-homoserine lactone + H2O
-
 Arthrobacter sp. N-decanoyl-L-homoserine
-
 ?
N-decanoyl-L-homoserine lactone + H2O
-
 Arthrobacter sp. IBN110 N-decanoyl-L-homoserine
-
 ?
N-hexanoyl-L-homoserine lactone + H2O
-
 Klebsiella pneumoniae N-hexanoyl-L-homoserine
-
 ?
N-hexanoyl-L-homoserine lactone + H2O
-
 Arthrobacter sp. N-hexanoyl-L-homoserine product analysis of recombinant enzyme expressed in Escherichia coli strain JM109 ?
N-hexanoyl-L-homoserine lactone + H2O
-
 Arthrobacter sp. IBN110 N-hexanoyl-L-homoserine product analysis of recombinant enzyme expressed in Escherichia coli strain JM109 ?
N-hexanoyl-L-homoserine lactone + H2O
-
 Klebsiella pneumoniae KCTC 2241 / ATCC 25306 N-hexanoyl-L-homoserine
-
 ?
N-octanoyl-L-homoserine lactone + H2O
-
 Arthrobacter sp. N-octanoyl-L-homoserine
-
 ?

Subunits

Subunits Comment Organism
? x * 30000, native enzyme, SDS-PAGE, x * 36000, recombinant enzyme, SDS-PAGE Arthrobacter sp.
More AhlD contains the conserved HXDH-H-D motif required for full enzyme activity Arthrobacter sp.

Synonyms

Synonyms Comment Organism
acyl homoserine degrading enzyme
-
 Arthrobacter sp.
AHL-degrading enzyme
-
 Arthrobacter sp.
AHL-degrading enzyme
-
 Klebsiella pneumoniae
AHLase
-
 Arthrobacter sp.
AHLase
-
 Klebsiella pneumoniae
AhlD
-
 Arthrobacter sp.
AhlK
-
 Klebsiella pneumoniae
N-acylhomoserine lactonase
-
 Arthrobacter sp.
N-acylhomoserine lactonase
-
 Klebsiella pneumoniae