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Literature summary for 3.1.1.76 extracted from

  • Seo, B.S.; Kim, d.o..Y.; Ni, Y.Y.; Son, K.H.; Park, H.Y.; Rhee, Y.H.
    Non-ionic polysorbate surfactants: alternative inducers of medium-chain-length poly(3-hydroxyalkanoates) (MCL-PHAs) for production of extracellular MCL-PHA depolymerases (2012), Biores. Technol., 121, 47-53.
    View publication on PubMed
Search for more literature for 3.1.1.76

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Pseudomonas alcaligenes
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26500
-
 native PAGE Pseudomonas alcaligenes

Organism

Organism UniProt Comment Textmining
Pseudomonas alcaligenes
-
-
-
Pseudomonas alcaligenes LB19
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native extracellular enzyme by hydrophobic interaction chromatography, gel filtration, and ion exchange chromatography Pseudomonas alcaligenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
poly(3-hydroxyhexanoate-co-3-hydroxyoctanoate-co-3-hydroxydecanoate) + H2O the substrate consists of Poly(4.4% 3-hydroxyhexanoate-co-86.0% 3-hydroxyoctanoate-co-9.6% 3-hydroxydecanoate) in a latex suspension Pseudomonas alcaligenes ?
-
 ?
poly(3-hydroxyhexanoate-co-3-hydroxyoctanoate-co-3-hydroxydecanoate) + H2O the substrate consists of Poly(4.4% 3-hydroxyhexanoate-co-86.0% 3-hydroxyoctanoate-co-9.6% 3-hydroxydecanoate) in a latex suspension Pseudomonas alcaligenes LB19 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 26500, SDS-PAGE Pseudomonas alcaligenes

Synonyms

Synonyms Comment Organism
MCL-PHA depolymerase
-
 Pseudomonas alcaligenes

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 in vivo assay at Pseudomonas alcaligenes
45
-
 in vitro assay at Pseudomonas alcaligenes

Expression

Organism Comment Expression
Pseudomonas alcaligenes the enzyme is induced by growth on Tween 20, overview up