Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | 10% activation at 10 mM | Bdellovibrio bacteriovorus | |
sodium cholate | 30% activation at 0.02 mM, 18% at 0.2 mM | Bdellovibrio bacteriovorus |
Application | Comment | Organism |
---|---|---|
synthesis | the enzyme migt be used as biocatalyst for the production of enantiopure hydroxyalkanoic acids and oligomers as building blocks for the synthesis of biobased polymers | Bdellovibrio bacteriovorus |
Cloned (Comment) | Organism |
---|---|
orf Bd3709, recombinant extracellular expression in different hypersecretor Tol-pal mutant strains of Escherichia coli and Pseudomonas putida, e.g. in Escherichia coli strain DH10B(pIZBd1) periplasm or Pseudomonas putida strain KT2442, expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)(pETBd1) | Bdellovibrio bacteriovorus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cetyltrimethylammonium bromide | 88% inhibition at 0.05 vol% | Bdellovibrio bacteriovorus | |
deoxycholate | 84% inhibition at 0.05 vol% | Bdellovibrio bacteriovorus | |
dithiothreitol | 45% inhibition at 10 mM DTT | Bdellovibrio bacteriovorus | |
additional information | not affected by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide treatment, and by 1 mM cyclodextrin | Bdellovibrio bacteriovorus | |
phenylmethylsulfonyl fluoride | 10% inhibition at 1 mM, 90% inhibition at 10 mM | Bdellovibrio bacteriovorus | |
SDS | 47% inhibition at 0.05 vol% | Bdellovibrio bacteriovorus | |
sodium cholate | 57% inhibition at 2 mM | Bdellovibrio bacteriovorus | |
Triton X-100 | 90% inhibition at 0.01 vol% | Bdellovibrio bacteriovorus | |
Tween 80 | 92% inhibition at 0.05 vol%, 79% inhibition at 0.005 vol% | Bdellovibrio bacteriovorus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Bdellovibrio bacteriovorus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no effect by 1 mM MgCl2 or CaCl2 | Bdellovibrio bacteriovorus | |
NaCl | a high ionic strength of 0.5 M NaCl in the reaction mixture is required for optimal activity | Bdellovibrio bacteriovorus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Bdellovibrio bacteriovorus | the enzyme is an endoexohydrolase that cleaves both large and small polyhydroxyalkanoate molecules, producing mainly dimers but also monomers and trimers. The enzyme specifically degrades medium-chain-length polyhydroxyalkanoates and is inactive toward short-chain-length polyhydroxyalkanoates like polyhydroxybutyrate | ? | - |
? | |
additional information | Bdellovibrio bacteriovorus HD100 | the enzyme is an endoexohydrolase that cleaves both large and small polyhydroxyalkanoate molecules, producing mainly dimers but also monomers and trimers. The enzyme specifically degrades medium-chain-length polyhydroxyalkanoates and is inactive toward short-chain-length polyhydroxyalkanoates like polyhydroxybutyrate | ? | - |
? | |
polyhydroxyalkanoate latex + H2O | Bdellovibrio bacteriovorus | - |
? | - |
? | |
polyhydroxyalkanoate latex + H2O | Bdellovibrio bacteriovorus HD100 | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bdellovibrio bacteriovorus | - |
orf Bd3709 | - |
Bdellovibrio bacteriovorus HD100 | - |
orf Bd3709 | - |
Purification (Comment) | Organism |
---|---|
recombinant extracellular enzyme by hydrophobic interaction chromatography, recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)(pETBd1) by nickel affinity chromatography | Bdellovibrio bacteriovorus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
55 | 560 | purified recombinant His-tagged enzme, pH 8.0, 37°C, different assay methods | Bdellovibrio bacteriovorus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme is an endoexohydrolase that cleaves both large and small polyhydroxyalkanoate molecules, producing mainly dimers but also monomers and trimers. The enzyme specifically degrades medium-chain-length polyhydroxyalkanoates and is inactive toward short-chain-length polyhydroxyalkanoates like polyhydroxybutyrate | Bdellovibrio bacteriovorus | ? | - |
? | |
additional information | the enzyme is an endoexohydrolase that cleaves both large and small polyhydroxyalkanoate molecules, producing mainly dimers but also monomers and trimers. The enzyme specifically degrades medium-chain-length polyhydroxyalkanoates and is inactive toward short-chain-length polyhydroxyalkanoates like polyhydroxybutyrate | Bdellovibrio bacteriovorus HD100 | ? | - |
? | |
polyhydroxyalkanoate latex + H2O | - |
Bdellovibrio bacteriovorus | ? | - |
? | |
polyhydroxyalkanoate latex + H2O | - |
Bdellovibrio bacteriovorus HD100 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
extracellular MCL-PHA depolymerase | - |
Bdellovibrio bacteriovorus |
MCL-PHA depolymerase | - |
Bdellovibrio bacteriovorus |
medium-chain-length polyhydroxyalkanoate depolymerase | - |
Bdellovibrio bacteriovorus |
PhaZBd | - |
Bdellovibrio bacteriovorus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Bdellovibrio bacteriovorus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 50 | activity range, highly active at 4-45°C, 29% of maximal activityy at 50°C | Bdellovibrio bacteriovorus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
- |
Bdellovibrio bacteriovorus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 11 | activity range, almost inactive at pH 12.0 | Bdellovibrio bacteriovorus |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the alpha/beta-hydrolase fold family | Bdellovibrio bacteriovorus |
additional information | the enzyme has a typical serine hydrolase catalytic triad, serine-histidine-aspartic acid | Bdellovibrio bacteriovorus |