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Literature summary for 3.1.1.75 extracted from

  • Sung, C.; Tachibana, Y.; Kasuya, K.
    Characterization of a thermolabile poly(3-hydroxybutyrate) depolymerase from the marine bacterium Shewanella sp. JKCM-AJ-6,1alpha (2016), Polym. Degrad. Stab., 129, 212-221 .
No PubMed abstract available
Search for more literature for 3.1.1.75

Inhibitors

Inhibitors Comment Organism Structure
dithiothreitol 5 mM, complete inhibition Shewanella sp. JKCM-AJ-6,1A
Fe2+ 1 mM less than 10% residual activity Shewanella sp. JKCM-AJ-6,1A
NaCl required for native enzyme, optimum activity at 0.5 M. Inhibitory for recombinant enzyme Shewanella sp. JKCM-AJ-6,1A
PMSF 5 mM, complete inhibition Shewanella sp. JKCM-AJ-6,1A
SDS 0.1%, complete inhibition Shewanella sp. JKCM-AJ-6,1A
Tween 20 0.1%, complete inhibition Shewanella sp. JKCM-AJ-6,1A

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl required for native enzyme, optimum activity at 0.5 M. Inhibitory for recombinant enzyme Shewanella sp. JKCM-AJ-6,1A

Organism

Organism UniProt Comment Textmining
Shewanella sp. JKCM-AJ-6,1A
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native and recombinant protein Shewanella sp. JKCM-AJ-6,1A

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15.34
-
 native enzyme, pH 7.4, 15°C Shewanella sp. JKCM-AJ-6,1A
58.35
-
 recombinant enzyme, pH 7.4, 15°C Shewanella sp. JKCM-AJ-6,1A

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
poly(3-hydroxybutanoate) + H2O recombinant enzyme has a stronger binding affinity for poly(3-hydroxybutanoate) than native enzyme Shewanella sp. JKCM-AJ-6,1A ?
-
 ?
poly(3-hydroxybutanoate-co-3-hydroxyvalerate) + H2O 18 mol% hydroxyvalerate content Shewanella sp. JKCM-AJ-6,1A ?
-
 ?
poly(ethylene succinate) + H2O
-
 Shewanella sp. JKCM-AJ-6,1A ?
-
 ?

Subunits

Subunits Comment Organism
? x * 70382, calculated from sequence, x * 47000, native enzyme, x * 70000, recombinant enzyme, SDS-PAGE Shewanella sp. JKCM-AJ-6,1A
More enzyme purified from Shewanella sp. lacks substrate binding domains which are present in the recombinant enzyme Shewanella sp. JKCM-AJ-6,1A

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
15
-
 stable up to Shewanella sp. JKCM-AJ-6,1A

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Shewanella sp. JKCM-AJ-6,1A

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 10.5
-
 Shewanella sp. JKCM-AJ-6,1A