Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.75 extracted from

  • Romen, F.; Reinhardt, S.; Jendrossek, D.
    Thermotolerant poly(3-hydroxybutyrate)-degrading bacteria from hot compost and characterization of the PHB depolymerase of Schlegelella sp. KB1a (2004), Arch. Microbiol., 182, 157-164.
    View publication on PubMed
Search for more literature for 3.1.1.75

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli DH5alpha Schlegelella sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Schlegelella sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49356
-
 1 * 49356, SDS-PAGE Schlegelella sp.
49360
-
 deduced from sequence, SDS-PAGE Schlegelella sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
poly[(R)-3-hydroxybutanoate]n + H2O Schlegelella sp.
-
 poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?
poly[(R)-3-hydroxybutanoate]n + H2O Schlegelella sp. KB1a
-
 poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?

Organism

Organism UniProt Comment Textmining
Schlegelella sp. Q5YEW3
-
-
Schlegelella sp. KB1a Q5YEW3
-
-

Purification (Commentary)

Purification (Comment) Organism
two chromatography steps, almost pure as determined by SDS-PAGE Schlegelella sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture fluid
-
 Schlegelella sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
poly[(R)-3-hydroxybutanoate]n + H2O
-
 Schlegelella sp. poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?
poly[(R)-3-hydroxybutanoate]n + H2O
-
 Schlegelella sp. KB1a poly[(R)-3-hydroxybutanoate]n-x + poly[(R)-3-hydroxybutanoate]x
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 49356, SDS-PAGE Schlegelella sp.

Synonyms

Synonyms Comment Organism
PHB depolymerase
-
 Schlegelella sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
76
-
-
 Schlegelella sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 stable for 1 h without loss of activity, above 75¡C rapid inactivation of the protein Schlegelella sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
 Schlegelella sp.

pH Range

pH Minimum pH Maximum Comment Organism
3 12
-
 Schlegelella sp.