Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Fusarium oxysporum |
Crystallization (Comment) | Organism |
---|---|
modeling of structure | Fusarium oxysporum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
4-nitrophenyl butanoate | pH 6.0, 40°C | Fusarium oxysporum | |
2.1 | - |
4-nitrophenyl laurate | pH 6.0, 40°C | Fusarium oxysporum | |
5.4 | - |
4-nitrophenyl acetate | pH 6.0, 40°C | Fusarium oxysporum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | recombinant protein | Fusarium oxysporum | 5737 | - |
periplasm | recombinant protein | Fusarium oxysporum | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fusarium oxysporum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | - |
Fusarium oxysporum | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl butanoate + H2O | - |
Fusarium oxysporum | 4-nitrophenol + butanoate | - |
? | |
4-nitrophenyl laurate | - |
Fusarium oxysporum | 4-nitrophenol + laurate | - |
? | |
additional information | the enzyme is capable of hydrolyzing polyethylene terephthalate model substrates and synthetic polymers | Fusarium oxysporum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 231740, calculated from sequence, x * 23000, SDS-PAGE | Fusarium oxysporum |
Synonyms | Comment | Organism |
---|---|---|
Cut5a | - |
Fusarium oxysporum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Fusarium oxysporum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.3 | - |
4-nitrophenyl laurate | pH 6.0, 40°C | Fusarium oxysporum | |
111.9 | - |
4-nitrophenyl butanoate | pH 6.0, 40°C | Fusarium oxysporum | |
121 | - |
4-nitrophenyl acetate | pH 6.0, 40°C | Fusarium oxysporum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Fusarium oxysporum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Fusarium oxysporum | calculated from sequence | - |
8.4 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.9 | - |
4-nitrophenyl laurate | pH 6.0, 40°C | Fusarium oxysporum | |
22.5 | - |
4-nitrophenyl acetate | pH 6.0, 40°C | Fusarium oxysporum | |
152.5 | - |
4-nitrophenyl butanoate | pH 6.0, 40°C | Fusarium oxysporum |