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Literature summary for 3.1.1.73 extracted from

  • Chi, D.; Giap, V.; Anh, L.; Nghi, D.
    Feruloyl esterase from Alternaria tenuissima that hydrolyses lignocellulosic material to release hydroxycinnamic acids (2017), Appl. Biochem. Microbiol., 53, 654-660 .
No PubMed abstract available
Search for more literature for 3.1.1.73

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0506
-
 methyl p-coumarate pH 6.0, 37°C Alternaria tenuissima
0.0614
-
 methyl ferulate pH 6.0, 37°C Alternaria tenuissima
0.1005
-
 ethyl ferulate pH 6.0, 37°C Alternaria tenuissima
0.1606
-
 methyl sinapate pH 6.0, 37°C Alternaria tenuissima

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Alternaria tenuissima
-
-

Organism

Organism UniProt Comment Textmining
Alternaria tenuissima
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purification from culture filtrate Alternaria tenuissima

Source Tissue

Source Tissue Comment Organism Textmining
culture filtrate
-
 Alternaria tenuissima
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11.2
-
 substrate methyl ferulate, pH 6.0, 37°C Alternaria tenuissima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ethyl ferulate + H2O
-
 Alternaria tenuissima ferulate + ethanol
-
 ?
methyl ferulate + H2O
-
 Alternaria tenuissima ferulate + methanol
-
 ?
methyl p-coumarate + H2O
-
 Alternaria tenuissima p-coumarate + methanol
-
 ?
methyl sinapate + H2O
-
 Alternaria tenuissima sinapate + methanol
-
 ?
additional information enzyme directly hydrolyzes native lignocellulosic raw materials. A synergistic effect is observed by enzymatic treatment in combination with cellulase/xylanase and acetyl esterase Alternaria tenuissima ?
-
 ?
wood meal + H2O
-
 Alternaria tenuissima ferulic acid + hydroxycinnamic acid + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 30300, SDS-PAGE Alternaria tenuissima

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45 50
-
 Alternaria tenuissima

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 8 h, 50% residual activity Alternaria tenuissima
60
-
 2 h, 50% residual activity Alternaria tenuissima

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1
-
 methyl sinapate pH 6.0, 37°C Alternaria tenuissima
7.5
-
 methyl p-coumarate pH 6.0, 37°C Alternaria tenuissima
19.9
-
 methyl ferulate pH 6.0, 37°C Alternaria tenuissima
25.9
-
 ethyl ferulate pH 6.0, 37°C Alternaria tenuissima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7
-
 Alternaria tenuissima

pH Range

pH Minimum pH Maximum Comment Organism
9
-
 85% of maximum activity Alternaria tenuissima

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
 2 h, 30% residual activity Alternaria tenuissima
7
-
 2 h, 97% residual activity Alternaria tenuissima
8
-
 2 h, 91% residual activity Alternaria tenuissima

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.3
-
 methyl sinapate pH 6.0, 37°C Alternaria tenuissima
150
-
 methyl p-coumarate pH 6.0, 37°C Alternaria tenuissima
260
-
 ethyl ferulate pH 6.0, 37°C Alternaria tenuissima
310
-
 methyl ferulate pH 6.0, 37°C Alternaria tenuissima