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Literature summary for 3.1.1.73 extracted from
- Biochemical characterization of a halotolerant feruloyl esterase from Actinomyces spp.: refolding and activity following thermal deactivation (2016), Appl. Microbiol. Biotechnol., 100, 1777-1787.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | 1 M, 115% of initial activity | Actinomyces sp. oral taxon 448 |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Actinomyces sp. oral taxon 448 |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | exprression in Escherichia coli with and without its putative signal peptide. The truncated form has less than 10% relative activity compared to the full length and is more prone to aggregation after purification | Actinomyces sp. oral taxon 448 |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.33 | - |
ethyl ferulate | pH 9.0, 30°C | Actinomyces sp. oral taxon 448 | |
| 2.79 | - |
methyl caffeate | pH 9.0, 30°C | Actinomyces sp. oral taxon 448 | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Actinomyces sp. oral taxon 448 | F9ECN6 | - |
- |
| Actinomyces sp. oral taxon 448 F0400 | F9ECN6 | - |
- |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| after treatment above the thermal denaturation temperature followed by cooling to room temperature, FaeI demonstrates spontaneous refolding into an active state | Actinomyces sp. oral taxon 448 |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 174.4 | - |
substrate methyl p-coumarate, pH 6.5, 30°C | Actinomyces sp. oral taxon 448 |
| 477.6 | - |
substrate methyl caffeate, pH 6.5, 30°C | Actinomyces sp. oral taxon 448 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| destarched wheat bran + H2O | - |
Actinomyces sp. oral taxon 448 | ferulic acid + ? | enzyme releases ferulic acid from destarched wheat bran when combined with a xylanase preparation | ? | |
| destarched wheat bran + H2O | - |
Actinomyces sp. oral taxon 448 F0400 | ferulic acid + ? | enzyme releases ferulic acid from destarched wheat bran when combined with a xylanase preparation | ? | |
| ethyl ferulate + H2O | - |
Actinomyces sp. oral taxon 448 | ethanol + ferulic acid | - |
? | |
| ethyl ferulate + H2O | - |
Actinomyces sp. oral taxon 448 F0400 | ethanol + ferulic acid | - |
? | |
| methyl caffeate + H2O | - |
Actinomyces sp. oral taxon 448 | methanol + caffeic acid | - |
? | |
| methyl caffeate + H2O | - |
Actinomyces sp. oral taxon 448 F0400 | methanol + caffeic acid | - |
? | |
| methyl p-coumarate + H2O | - |
Actinomyces sp. oral taxon 448 | methanol + p-coumarate | - |
? | |
| methyl p-coumarate + H2O | - |
Actinomyces sp. oral taxon 448 F0400 | methanol + p-coumarate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CinI | - |
Actinomyces sp. oral taxon 448 |
| FaeI | - |
Actinomyces sp. oral taxon 448 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Actinomyces sp. oral taxon 448 |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 41.5 | - |
standard buffer, thermal unfolding temperature | Actinomyces sp. oral taxon 448 |
| 74 | - |
presence of 2.5 M sodium malonate, thermal unfolding temperature | Actinomyces sp. oral taxon 448 |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 10.53 | - |
ethyl ferulate | pH 9.0, 30°C | Actinomyces sp. oral taxon 448 | |
| 278 | - |
methyl caffeate | pH 9.0, 30°C | Actinomyces sp. oral taxon 448 | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Actinomyces sp. oral taxon 448 |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
more than 90% of maximum activity | Actinomyces sp. oral taxon 448 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7.9 | - |
ethyl ferulate | pH 9.0, 30°C | Actinomyces sp. oral taxon 448 | |
| 99.6 | - |
methyl caffeate | pH 9.0, 30°C | Actinomyces sp. oral taxon 448 | |
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