Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Aspergillus niger |
Protein Variants | Comment | Organism |
---|---|---|
D93G/S187F | about 13fold increaase in catalytic turnover efficiency | Aspergillus niger |
D93G/S187F/C235I | specific activity similar to wild-type, significant increase in thermal stability | Aspergillus niger |
D93G/S187F/C235N | specific activity similar to wild-type, significant increase in thermal stability | Aspergillus niger |
D93G/S187F/C235R | specific activity similar to wild-type, significant increase in thermal stability | Aspergillus niger |
D93G/S187F/C235S | specific activity similar to wild-type, significant increase in thermal stability | Aspergillus niger |
D93G/S187F/C235V | specific activity similar to wild-type, significant increase in thermal stability | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | O42807 | - |
- |
Renatured (Comment) | Organism |
---|---|
the thermal unfolding of the parental enzyme is irreversible on all the tested conditions, while that of the Cys235 mutants is reversible, and their ability to refold is highly dependent on the denaturing temperature. Mutants denatured at 75°C are able to efficiently reverse the unfolding to regain native structure during the cooling process | Aspergillus niger |
Synonyms | Comment | Organism |
---|---|---|
FAEA | - |
Aspergillus niger |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the unfolding of the parental enzyme is irreversible on all the tested conditions, while that of the Cys235 mutants is reversible, and their ability to refold is highly dependent on the denaturing temperature. Mutants denatured at 75°C are able to efficiently reverse the unfolding to regain native structure during the cooling process | Aspergillus niger |
60 | - |
wild-type half-life 8.3 min, half-lifes of mutants D93G/S187/C235I, D93G/S187F/C235N, D93G/S187F/C235S, D93G/S187F/C235V, D93G/S187F/C235R 9.8 to 14 min | Aspergillus niger |
70 | - |
wild-type half-life 1.6 min, half-lifes of mutants D93G/S187/C235I, D93G/S187F/C235N, D93G/S187F/C235S, D93G/S187F/C235V, D93G/S187F/C235R 21 to 63 min | Aspergillus niger |